Joester W, Joester K E, van Dorp B, Hofschneider P H
Nucleic Acids Res. 1978 Aug;5(8):3043-55. doi: 10.1093/nar/5.8.3043.
Two DNA-dependent DNA-polymerases (E.C. 2.7.7.7) are partially purified from the high speed supernatant of mechanically disrupted hyphae of Neurospora crassa WT 74A. Some properties such as temperature and pH optimum and theoptimal concentrations for Mg2+, Zn2+, NH4+ and Na+ are very similar. On the other hand these enzymes show different properties on ion-exchange columns, are well distinguished by molecular weight (147 000 d and 110 000 d for A and B resp.) and the stimulation by K+ differs (K+ optimum for A: 5-70 mM and for B: 45 mM). Mn2+ and Zn2+ inhibit incorporation of deoxyribonucleoside monophosphates between 70 and 90%. Our best preparations so far have specific activities of 13 200 units/mg protein for A and 12 000 units/mg protein for B.
从粗糙脉孢菌野生型74A机械破碎菌丝的高速上清液中部分纯化出两种依赖DNA的DNA聚合酶（E.C. 2.7.7.7）。它们的一些特性，如最适温度和pH值以及Mg2+、Zn2+、NH4+和Na+的最佳浓度非常相似。另一方面，这些酶在离子交换柱上表现出不同的特性，通过分子量可以很好地区分（A和B的分子量分别为147000 d和110000 d），并且K+的刺激作用也不同（A的K+最佳浓度为5 - 70 mM，B为45 mM）。Mn2+和Zn2+抑制脱氧核糖核苷单磷酸的掺入达70%至90%。到目前为止，我们制备的最佳样品中，A的比活性为13200单位/毫克蛋白质，B为12000单位/毫克蛋白质。
