James A C, Dean M D, McMahon M E, Ballard J W O
Department of Biology, University of Iowa, Iowa City, IA 52242, USA.
Heredity (Edinb). 2002 Mar;88(3):182-9. doi: 10.1038/sj.hdy.6800025.
The bacterial symbiont Wolbachia can cause cytoplasmic incompatibility in Drosophila simulans flies: if an infected male mates with an uninfected female, or a female with a different strain of Wolbachia, there can be a dramatic reduction in the number of viable eggs produced. Here we explore the dynamics associated with double and single Wolbachia infections in New Caledonia. Doubly infected females were compatible with all males in the population, explaining the high proportion of doubly infected flies. In this study, males that carry only wHa or wNo infections showed reduced incompatibility when mated to uninfected females, compared with previous reports. These data suggest that either the DNA of these bacterial isolates have diverged from those previously collected, or the genetic background of the host has lead to a reduction in the phenotype of incompatibility. Mitochondrial sequence polymorphism at two sites within the host genome was assayed to investigate population structure related to infection types. There was no correlation between sequence polymorphism and infection type suggesting that double infections are the stable type, with singly infected and uninfected flies arising from stochastic segregation of bacterial strains. Finally, we discuss the nomenclature of Wolbachia strain designation.
如果受感染的雄性与未受感染的雌性交配，或者雌性与不同菌株的沃尔巴克氏体交配，所产 viable 卵的数量可能会大幅减少。在这里，我们探讨了新喀里多尼亚双重和单一沃尔巴克氏体感染相关的动态变化。双重感染的雌性与种群中的所有雄性都具有亲和性，这解释了双重感染果蝇的高比例。在本研究中，与之前的报告相比，仅携带 wHa 或 wNo 感染的雄性与未受感染的雌性交配时，不亲和性降低。这些数据表明，要么这些细菌分离株的 DNA 与之前收集的不同，要么宿主的遗传背景导致了不亲和表型的降低。对宿主基因组内两个位点的线粒体序列多态性进行了分析，以研究与感染类型相关的种群结构。序列多态性与感染类型之间没有相关性，这表明双重感染是稳定类型，单一感染和未感染的果蝇是由细菌菌株的随机分离产生的。最后，我们讨论了沃尔巴克氏体菌株命名法。
