Troussard X, Avet-Loiseau H, Macro M, Mellerin M P, Malet M, Roussel M, Sola B
Laboratoire d'Hématologie, CHU Caen, France, and UPRES-EA 2128, UFR de Médecine, Caen, France.
Hematol J. 2000;1(3):181-5. doi: 10.1038/sj.thj.6200025.
Chromosomal abnormalities are detected in 50 to 70% of patients with multiple myeloma (MM). By conventional cytogenetic analysis, a t(11;14)(q13;q32) is observed at a frequency of 3 to 14%.
To demonstrate a cyclin D1 expression in MM patients or MM cell lines, 14 patients with multiple myeloma (MM) and nine human multiple myeloma cell lines (HMCL) were screened by a competitive RT-PCR and/or Northern blot analysis for cyclin D1 expression. Furthermore, we screened 10 MM patients with FISH to demonstrate a relationship between the cyclin D1 expression and the presence of the t(11;14).
Five HMCL had a cyclin D1 overexpression: three of them had a t(11;14)(q13;q32) and two had extra copies of chromosome 11. A cyclin D1 expression was found at diagnosis in seven out of 14 untreated MM patients (50%). Out of 14 MM patients, FISH studies were performed in 10 patients. A t(11;14) was detected in three out of 10 patients and extra copies of chromosome 11 were found in two additional patients.
Cyclin D1 expression is a common event in MM patients (50%) and is associated either with a t(11;14)(q13;q32) or extra copies of chromosome 11. The prognostic role of the cyclin D1 expression and the level of this expression, as compared to other B-cell chronic lymphoproliferative disorders such as mantle cell lymphoma or hairy cell leukemia, remains to be determined in the pathogenesis of multiple myeloma.
在50%至70%的多发性骨髓瘤（MM）患者中可检测到染色体异常。通过传统细胞遗传学分析，观察到t(11;14)(q13;q32)的频率为3%至14%。
为了证明MM患者或MM细胞系中细胞周期蛋白D1的表达，通过竞争性逆转录聚合酶链反应（RT-PCR）和/或Northern印迹分析，对14例多发性骨髓瘤（MM）患者和9个人类多发性骨髓瘤细胞系（HMCL）进行细胞周期蛋白D1表达筛查。此外，我们对10例MM患者进行荧光原位杂交（FISH）检测，以证明细胞周期蛋白D1表达与t(11;14)存在之间的关系。
5个HMCL存在细胞周期蛋白D1过表达：其中3个有t(11;14)(q13;q32)，2个有11号染色体额外拷贝。在14例未经治疗的MM患者中，7例（50%）在诊断时发现有细胞周期蛋白D1表达。在14例MM患者中，对10例进行了FISH研究。10例患者中有3例检测到t(11;14)，另外2例发现有11号染色体额外拷贝。
细胞周期蛋白D1表达在MM患者中是常见事件（50%），并且与t(11;14)(q13;q32)或11号染色体额外拷贝相关。与其他B细胞慢性淋巴细胞增殖性疾病如套细胞淋巴瘤或毛细胞白血病相比，细胞周期蛋白D1表达及其表达水平在多发性骨髓瘤发病机制中的预后作用仍有待确定。
