Alter Galit, Merchant Alefia, Tsoukas Christos M, Rouleau Danielle, LeBlanc Roger P, Côté Pierre, Baril Jean-Guy, Thomas Réjean, Nguyen Vinh-Kim, Sékaly Rafik-Pierre, Routy Jean-Pierre, Bernard Nicole F
McGill University Health Centre, Montreal, Quebec, Canada.
J Infect Dis. 2002 Mar 15;185(6):755-65. doi: 10.1086/339338. Epub 2002 Feb 20.
The interferon-gamma ELISPOT assay was used to assess and compare the magnitude and breadth of human immunodeficiency virus (HIV)-specific CD8 T cell responses in treatment-naive subjects during the first year of HIV primary infection and during the chronic phase of infection. Twenty-five subjects tested within a year of exposure to HIV resulting in seroconversion and 20 subjects with chronic infection were screened for HIV peptide-specific activity by stimulating peripheral blood mononuclear cells with a panel of 5-21 HLA class I-restricted HIV peptides (mean, 11.2 +/- 3.5 HIV peptides). There was a significant correlation between the magnitude and breadth of HIV-specific effector responses and time elapsed from exposure (r=0.63 for magnitude vs. time and r=0.64 for breadth vs. time; P<.02, paired t test). Maximal breadth of the HIV gene product reactivity was achieved within 2 months of exposure for Nef-specific responses and by 4 months of exposure for responses directed to Env, Gag, and reverse transcriptase.
采用干扰素-γ酶联免疫斑点试验（ELISPOT），评估并比较初次感染人类免疫缺陷病毒（HIV）的未治疗受试者在HIV原发感染第一年及慢性感染期HIV特异性CD8 T细胞反应的强度和广度。对25名在接触HIV导致血清转化后一年内接受检测的受试者以及20名慢性感染受试者，通过用一组5 - 21种HLA I类限制性HIV肽（平均11.2±3.5种HIV肽）刺激外周血单个核细胞，筛查HIV肽特异性活性。HIV特异性效应反应的强度和广度与接触后经过的时间之间存在显著相关性（强度与时间的相关系数r = 0.63，广度与时间的相关系数r = 0.64；配对t检验，P<0.02）。针对Nef的反应在接触后2个月内达到HIV基因产物反应性的最大广度，针对Env、Gag和逆转录酶的反应在接触后4个月达到最大广度。
