Whitaker Thomas B, Hagler Winston M, Giesbrecht Francis G, Johansson Anders S
U.S. Department of Agriculture, Agricultural Research Service, North Carolina State University, Raleigh 27695-7625, USA.
Adv Exp Med Biol. 2002;504:73-83. doi: 10.1007/978-1-4615-0629-4_8.
The variability associated with testing wheat for deoxynivalenol (DON) was measured using a 0.454 kg sample, a Romer mill, 25 g of comminuted subsample and the Romer Fluoroquant analytical method. The total variability was partitioned into sampling, sample preparation, and analytical variability components. Each variance component was found to be a function of the DON concentration and equations were developed to predict each variance component using regression techniques. The effects of sample size, subsample size, and number of aliquots on reducing the variability of the DON test procedure were also determined. Using the test procedure described above, the coefficient of variation (CV) associated with testing wheat at 5 ppm DON was found to be 13.4%. The CVs associated with sampling, sample preparation, and analysis were 6.3, 10.0, and 6.3%, respectively. The sample variations associated with testing wheat are relatively small when compared to CVs associated with testing other commodities for other mycotoxins such as aflatoxin in peanuts. Even with the use of a small sample size (0.454 kg), the sampling variation was not the largest source of error as found in other mycotoxin test procedures.
使用0.454千克的样品、罗默磨粉机、25克粉碎后的子样品以及罗默荧光定量分析方法，对小麦脱氧雪腐镰刀菌烯醇（DON）检测的变异性进行了测定。总变异性被划分为采样、样品制备和分析变异性成分。发现每个方差成分都是DON浓度的函数，并使用回归技术建立了预测每个方差成分的方程。还确定了样品大小、子样品大小和等分份数对降低DON检测程序变异性的影响。使用上述检测程序，发现在5 ppm DON水平下检测小麦时的变异系数（CV）为13.4%。与采样、样品制备和分析相关的CV分别为6.3%、10.0%和6.3%。与检测小麦相关的样品变异与检测其他商品中其他霉菌毒素（如花生中的黄曲霉毒素）的CV相比相对较小。即使使用小样品量（0.454千克），采样变异也不像其他霉菌毒素检测程序那样是最大的误差来源。
