Whitaker T B, Hagler W M, Giesbrecht F G, Johansson A S
US Department of Agriculture, Agricultural Research Service, North Carolina State University, Raleigh 27695-7625, USA.
J AOAC Int. 2000 Sep-Oct;83(5):1285-92.
The variability associated with testing wheat for deoxynivalenol (DON) was measured using a 0.454 kg sample, Romer mill, 25 g comminuted subsample, and the Romer Fluoroquant analytical method. The total variability was partitioned into sampling, sample preparation, and analytical variability components. Each variance component was a function of the DON concentration and equations were developed to predict each variance component using regression techniques. The effect of sample size, subsample size, and number of aliquots on reducing the variability of the DON test procedure was also determined. For the test procedure, the coefficient of variation (CV) associated with testing wheat at 5 ppm was 13.4%. The CVs associated with sampling, sample preparation, and analysis were 6.3, 10.0, and 6.3%, respectively. For the sample variation, a 0.454 kg sample was used; for the sample preparation variation, a Romer mill and a 25 g subsample were used; for the analytical variation, the Romer Fluoroquant method was used. The CVs associated with testing wheat are relatively small compared to the CV associated with testing other commodities for other mycotoxins, such as aflatoxin in peanuts. Even when the small sample size of 0.454 kg was used, the sampling variation was not the largest source of error as found in other mycotoxin test procedures.
使用0.454千克样品、罗默磨粉机、25克粉碎子样品以及罗默荧光定量分析法，测定了小麦脱氧雪腐镰刀菌烯醇（DON）检测的变异性。总变异性被划分为采样、样品制备和分析变异性成分。每个方差成分都是DON浓度的函数，并使用回归技术建立了预测每个方差成分的方程。还确定了样品大小、子样品大小和等分份数对降低DON检测程序变异性的影响。对于该检测程序，在5 ppm下检测小麦的变异系数（CV）为13.4%。与采样、样品制备和分析相关的CV分别为6.3%、10.0%和6.3%。对于样品变异，使用0.454千克样品；对于样品制备变异，使用罗默磨粉机和25克子样品；对于分析变异，使用罗默荧光定量法。与检测其他商品中其他霉菌毒素（如花生中的黄曲霉毒素）的CV相比，检测小麦的CV相对较小。即使使用0.454千克的小样品量，采样变异也不像其他霉菌毒素检测程序中那样是最大的误差来源。
