Kaiser M G, Lakshmanan N, Wing T, Lamont S J
Department of Animal Science, Iowa State University, Ames 50011-3150, USA.
Avian Dis. 2002 Jan-Mar;46(1):25-31. doi: 10.1637/0005-2086(2002)046[0025:SESEBI]2.0.CO;2.
The relationship between antibody response to Salmonella enteritidis vaccine and internal organ burden of S. enteritidis is not fully understood. The genetic relationship, therefore, between postchallenge S. enteritidis burden and antibody response to S. enteritidis vaccine was determined in broiler breeder chicks. Sibling chicks from a broiler breeder male line were either inoculated with a pathogenic S. enteritidis or vaccinated with a commercial S. enteritidis vaccine. Spleen, liver, cecal wall, and cecal content samples from S. enteritidis-challenged chicks (n = 120) were cultured for enumeration of bacteria. Unchallenged chicks (n = 314) were vaccinated at 11 days of age, and serum samples were taken at 10 days postvaccination. Antibody response to vaccination and number of S. enteritidis in cecal content cultures were negatively correlated (-0.772), demonstrating that genetic potential for greater antibody response to S. enteritidis vaccine is associated with lesser S. enteritidis bacterial burden in cecal content of broiler breeder chicks. The findings suggest that genetic selection for vaccine antibody responsiveness can lower bacterial burden in the gut lumenal content and, thus, potentially reduce contamination of poultry products at processing.
对肠炎沙门氏菌疫苗的抗体反应与肠炎沙门氏菌的内脏器官负荷之间的关系尚未完全明确。因此，在肉种鸡雏鸡中确定了攻毒后肠炎沙门氏菌负荷与对肠炎沙门氏菌疫苗的抗体反应之间的遗传关系。来自肉种鸡雄性品系的同胞雏鸡，一部分接种致病性肠炎沙门氏菌，另一部分接种市售肠炎沙门氏菌疫苗。对受肠炎沙门氏菌攻毒的雏鸡（n = 120）的脾脏、肝脏、盲肠壁和盲肠内容物样本进行培养，以计数细菌。未攻毒的雏鸡（n = 314）在11日龄时接种疫苗，并在接种疫苗后10天采集血清样本。对疫苗接种的抗体反应与盲肠内容物培养物中肠炎沙门氏菌的数量呈负相关（-0.772），表明对肠炎沙门氏菌疫苗产生更大抗体反应的遗传潜力与肉种鸡雏鸡盲肠内容物中较低的肠炎沙门氏菌细菌负荷相关。研究结果表明，针对疫苗抗体反应性进行遗传选择可以降低肠道腔内容物中的细菌负荷，从而有可能减少家禽产品在加工过程中的污染。
