Berthelot-Hérault Florence, Mompart Florence, Zygmunt Michel S, Dubray Gérard, Duchet-Suchaux Marion
Institut National de la Recherche Agronomique, Pathologie Infectieuse et Immunologie, Centre de Tours, 37380 Nouzilly, France.
Vet Immunol Immunopathol. 2003 Nov 15;96(1-2):43-52. doi: 10.1016/s0165-2427(03)00155-7.
Salmonella frequently causes human foodborne infections. Contaminated products from poultry infected with Salmonella enteritidis are mainly involved. This serovar is able to colonize the gastrointestinal tract and generally produces a chronic asymptomatic carrier state in poultry, except in very young birds. We have developed a model of S. enteritidis carriage in chicks and found that four chicken lines, B13, L2, PA12 and Y11 differ in their cecal colonization by S. enteritidis, whereas their systemic organs are similarly infected. We have monitored the serum and gut antibody responses of these four lines to S. enteritidis for 9 weeks post inoculation (pi). We confirm that S. enteritidis infected the spleens of the four chicken lines similarly, and that it often colonized the ceca at levels significantly higher in B13 and L2 chicks than those of the PA12 and Y11 chicks. The serum IgM and IgG antibody responses were high and the serum IgA antibody responses low. In contrast, the intestinal secretions contained mostly IgA antibodies. The serum IgM antibody values of the four chicken lines were similar. However, the B13 and L2 chicks often had significantly higher serum IgG and IgA antibody responses than PA12 and Y11 chicks. Only the B13 and L2 chicks showed high, persistent levels of IgA antibody in intestinal secretions. These results suggest that most antibody responses are related to cecal colonization by S. enteritidis. They also indicate that factors other than the antibody levels are involved in the control of this colonization.
沙门氏菌经常导致人类食源性感染。主要涉及感染肠炎沙门氏菌的家禽所产的受污染产品。除了非常年幼的禽类外，这种血清型能够在胃肠道定植，并通常在家禽中产生慢性无症状携带状态。我们建立了雏鸡肠炎沙门氏菌携带模型，发现四个品系的鸡，即B13、L2、PA12和Y11，在盲肠被肠炎沙门氏菌定植方面存在差异，而它们的全身器官受到的感染相似。我们对接种后9周内这四个品系对肠炎沙门氏菌的血清和肠道抗体反应进行了监测。我们证实，肠炎沙门氏菌对四个品系鸡脾脏的感染情况相似，并且它在B13和L2雏鸡盲肠中的定植水平通常显著高于PA12和Y11雏鸡。血清IgM和IgG抗体反应较高，而血清IgA抗体反应较低。相比之下，肠道分泌物中主要含有IgA抗体。四个品系鸡的血清IgM抗体值相似。然而，B13和L2雏鸡的血清IgG和IgA抗体反应通常显著高于PA12和Y11雏鸡。只有B13和L2雏鸡的肠道分泌物中显示出高水平且持续的IgA抗体。这些结果表明，大多数抗体反应与肠炎沙门氏菌在盲肠的定植有关。它们还表明，除抗体水平外的其他因素也参与了对这种定植的控制。
