Hasenstein Jason R, Zhang Guolong, Lamont Susan J
Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, IA 50011-3150, USA.
Infect Immun. 2006 Jun;74(6):3375-80. doi: 10.1128/IAI.00027-06.
Gallinacins in poultry are functional equivalents of mammalian beta-defensins, which constitute an integral component of the innate immune system. Salmonella enterica serovar Enteritidis is a gram-negative bacterium that negatively affects both human and animal health. To analyze the association of genetic variations of the gallinacin genes with the phenotypic response to S. enterica serovar Enteritidis, an F1 population of chickens was created by crossing four outbred broiler sires to dams of two highly inbred lines. The F1 chicks were evaluated for bacterial colonization after pathogenic S. enterica serovar Enteritidis inoculation and for circulating antibody levels after inoculation with S. enterica serovar Enteritidis bacterin vaccine. Five candidate genes were studied, including gallinacins 2, 3, 4, 5, and 7. Gene fragments were sequenced from the founder individuals of the resource population, and a mean of 13.2 single-nucleotide polymorphisms (SNP) per kilobase was identified. One allele-defining SNP per gene was utilized to test for statistical associations of sire alleles with progeny response to S. enterica serovar Enteritidis. Among the five gallinacin genes evaluated, the Gal3 and Gal7 SNPs in broiler sires were found to be associated with antibody production after S. enterica serovar Enteritidis vaccination. Utilization of these SNPs as molecular markers for the response to S. enterica serovar Enteritidis may result in the enhancement of the immune response in poultry.
家禽中的加里纳辛与哺乳动物的β-防御素功能相当，而β-防御素是先天免疫系统的一个组成部分。肠炎沙门氏菌肠炎血清型是一种革兰氏阴性细菌，对人类和动物健康均有负面影响。为了分析加里纳辛基因的遗传变异与对肠炎沙门氏菌肠炎血清型表型反应之间的关联，通过将4只远交肉鸡父本与2个高度近交品系的母本杂交，创建了一个鸡的F1群体。对F1代雏鸡接种致病性肠炎沙门氏菌肠炎血清型后评估细菌定植情况，并在接种肠炎沙门氏菌肠炎血清型菌苗疫苗后评估循环抗体水平。研究了5个候选基因，包括加里纳辛2、3、4、5和7。从资源群体的奠基个体中对基因片段进行测序，平均每千碱基鉴定出13.2个单核苷酸多态性（SNP）。每个基因使用一个定义等位基因的SNP来测试父本等位基因与后代对肠炎沙门氏菌肠炎血清型反应的统计关联。在所评估的5个加里纳辛基因中，发现肉鸡父本中的Gal3和Gal7 SNP与接种肠炎沙门氏菌肠炎血清型疫苗后的抗体产生有关。将这些SNP用作对肠炎沙门氏菌肠炎血清型反应的分子标记可能会增强家禽的免疫反应。
