Yoshioka Y, Emori M, Ikekawa T, Fukuoka F
Carbohydr Res. 1975 Sep;43(2):305-20. doi: 10.1016/s0008-6215(00)83495-3.
Isolation of an antitumor component from polysaccharide fraction A5 of some Basidiomyces was achieved by column chromatography on Sephadex G-200. A detection method based on the specific rotatory characteristics of the polysaccharide was applied to estimate components in effluent fractions from the chromatography, and it was confirmed that a series of eluates having similar specific rotation was made up of homogeneous polysaccharide. Three components (H51, H52, and H53) were isolated, in chromatographically pure state, from fraction A5. Component H51 consisted of a skeleton of beta-(1 leads to 3)-linked glucose residues, probably having branches of galactose and mannose residues, and also containing acidic sugars. Component H53 had a main structure similarly consisting of beta-(1 leads to 3)-linked glucose residues and a larger proportion of acidic sugar than H51. Component H52 was a heteropolysaccharide made up of alpha-linked galactose and mannose residues. Components H51 and H53 had a higher and a lower molecular weight, respectively, than H52. The only antitumor-active component was H51.
通过在葡聚糖凝胶G - 200上进行柱色谱法，从某些担子菌的多糖组分A5中分离出一种抗肿瘤成分。基于该多糖的旋光特性的检测方法被用于估计色谱流出级分中的成分，并且证实具有相似比旋度的一系列洗脱液由均一的多糖组成。从组分A5中以色谱纯态分离出三种成分（H51、H52和H53）。成分H51由β-(1→3)连接的葡萄糖残基骨架组成，可能具有半乳糖和甘露糖残基分支，并且还含有酸性糖。成分H53具有类似的主要结构，同样由β-(1→3)连接的葡萄糖残基组成，且酸性糖的比例比H51大。成分H52是由α连接的半乳糖和甘露糖残基组成的杂多糖。成分H51和H53的分子量分别比H52高和低。唯一具有抗肿瘤活性的成分是H51。
