Li Xiaoyu, Wang Zhenyu, Wang Lu, Walid Elfalleh, Zhang Hua
Harbin Institute of Technology, 73 Huanghe Road, Nangang District, Harbin 150090, China.
Northeast Forestry University, 26 Hexing Road, Xiangfang District, Harbin 150040, China.
Int J Mol Sci. 2012;13(5):5801-5817. doi: 10.3390/ijms13055801. Epub 2012 May 15.
Polysaccharides were extracted from eight kinds of Chinese mushrooms using three solvents and were evaluated for their total carbohydrate, polyphenolic and protein contents, and antioxidant and anti-proliferation activities. The results suggested that all the polysaccharides had significant antioxidant capacities (EC(50) ranged from 1.70 ± 0.42 to 65.98 ± 1.74 μM TE/g crude polysaccharide inhibition of ABTS(+), EC(50) ranged from 5.06 ± 0.12 to 127.38 ± 1.58 mg VCE/g CP scavenging of OH· and EC(50) ranged from 0.70 ± 0.04 to 33.54 ± 0.49 mg VCE/g CP inhibition of lipid peroxidation) (TE: trolox equivalent; VCE: VC equivalent; CP: crude polysaccharide). The acid extracts of Russula vinosa Lindblad had the highest ABTS(+) scavenging activity. Aqueous extracts of Dictyophora indusiata and Hohenbuehelia serotina possessed, respectively, the highest OH· scavenging capacity and ability to inhibit lipid peroxidation. Mushroom extracts also inhibited proliferation of HeLa and HepG2 cells in a dose-dependent manner. These results indicate that the mushroom polysaccharides might be potential antioxidant resources.
采用三种溶剂从八种中国蘑菇中提取多糖，并对其总碳水化合物、多酚和蛋白质含量以及抗氧化和抗增殖活性进行评估。结果表明，所有多糖均具有显著的抗氧化能力（ABTS(+)抑制的EC(50)范围为1.70±0.42至65.98±1.74μM TE/g粗多糖，OH·清除的EC(50)范围为5.06±0.12至127.38±1.58mg VCE/g CP，脂质过氧化抑制的EC(50)范围为0.70±0.04至33.54±0.49mg VCE/g CP）（TE：Trolox当量；VCE：VC当量；CP：粗多糖）。红菇的酸提取物具有最高的ABTS(+)清除活性。竹荪和晚生 Hohenbuehelia serotina的水提取物分别具有最高的OH·清除能力和抑制脂质过氧化的能力。蘑菇提取物还以剂量依赖性方式抑制HeLa和HepG2细胞的增殖。这些结果表明，蘑菇多糖可能是潜在的抗氧化资源。
