Sapsford Kim E, Charles Paul T, Patterson Charles H, Ligler Frances S
Center for Bioresource Development, George Mason University, Fairfax, Virginia 22030-4444, USA.
Anal Chem. 2002 Mar 1;74(5):1061-8. doi: 10.1021/ac0157268.
The ability of a fluorescence-based array biosensor to measure and quantify the binding of an antigen to an immobilized antibody has been demonstrated using the four different immunoassay formats: direct, competitive, displacement, and sandwich. A patterned array of antibodies specific for 2,4,6-trinitrotoluene (TNT) immobilized onto the surface of a planar waveguide and used to measure signals from different antigen concentrations simultaneously. For direct, competitive, and displacement assays, which are one-step assays, measurements were obtained in real time. Dose-response curves were calculated for all four assay formats, demonstrating the array biosensor's ability to quantify the amount of antigen present in solution.
基于荧光的阵列生物传感器通过直接、竞争、置换和夹心这四种不同的免疫测定形式，已证明其能够测量和量化抗原与固定化抗体的结合。将针对2,4,6-三硝基甲苯（TNT）的特异性抗体的图案化阵列固定在平面波导表面，用于同时测量来自不同抗原浓度的信号。对于直接、竞争和置换测定这些一步测定法，实时获得了测量结果。计算了所有四种测定形式的剂量反应曲线，证明了阵列生物传感器能够量化溶液中存在的抗原量。
