Taitt C R, Golden J P, Shubin Y S, Shriver-Lake L C, Sapsford K E, Rasooly A, Ligler F S
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375-5348, USA.
Microb Ecol. 2004 Feb;47(2):175-85. doi: 10.1007/s00248-003-1011-1. Epub 2004 Feb 9.
The Multi-Analyte Array Biosensor (MAAB) has been developed at the Naval Research Laboratory (NRL) with the goal of simultaneously detecting and identifying multiple target agents in complex samples with minimal user manipulation. This paper will focus on recent improvements in the biochemical and engineering aspects of this instrument. These improvements have enabled the expansion of the repertoire of analytes detected to include Salmonella typhimurium and Listeria monocytogenes, and also expanded the different sample matrices tested. Furthermore, all components of the biochemical assays could be prepared well in advance of sample testing, resulting in a "plug-and-play" methodology. Simultaneous detection of three toxins (ricin, staphylococcal enterotoxin B, and cholera toxin) was demonstrated using a novel fluidics cube module that limits the number of manipulations to only the initial sample loading. This work demonstrates the utility of the MAAB for rapid analysis of complex samples with multianalyte capability, with a minimum of operator manipulations required for either sample preparation or final analysis.
海军研究实验室（NRL）开发了多分析物阵列生物传感器（MAAB），其目标是在用户操作最少的情况下，同时检测和识别复杂样品中的多种目标物。本文将重点介绍该仪器在生化和工程方面的最新改进。这些改进使得检测的分析物种类得以扩展，包括鼠伤寒沙门氏菌和单核细胞增生李斯特菌，同时也扩大了测试的不同样品基质范围。此外，生化检测的所有组件都可以在样品测试之前提前准备好，从而形成一种“即插即用”的方法。使用一种新型流体立方模块展示了同时检测三种毒素（蓖麻毒素、葡萄球菌肠毒素B和霍乱毒素）的能力，该模块将操作次数限制为仅初始样品加载。这项工作证明了MAAB在具有多分析物能力的复杂样品快速分析中的实用性，样品制备或最终分析所需操作人员操作最少。
