College of Chemical Engineering and Technology, Tianshui Normal University, 741001, Tianshui, Gansu, China.
J Fluoresc. 2023 Sep;33(5):1981-1993. doi: 10.1007/s10895-023-03207-1. Epub 2023 Mar 18.
Picric acid (PA) is a powerful nitro-aromatic explosive that harms the environment and human health. Developing non-toxic and low-cost sensors for the rapid detection of PA is essential. An environment-friendly fluorescent probe for PA detection is designed based on carbon dots (CDs) directly separated from edible soy sauce by silica gel column chromatography. Neither organic reagents nor heating process was needed to prepare CDs. The obtained CDs exhibit bright blue fluorescence, good water solubility, and photostability. The fluorescent probe for PA was developed according to the CD's fluorescence can be significantly quenched via the inner filter effect between CDs and PA. The linear range was 0.2-24 µM with a limit of detection of 70 nM. This proposed method was successfully employed to detect PA in the real water samples with satisfactory recoveries between 98.0-104.0%. Moreover, the CDs were suitable for fluorescence imaging of HeLa cells owing to their low toxicity and good biocompatibility.
苦味酸（PA）是一种强大的硝基芳香族爆炸物，对环境和人体健康有害。开发用于快速检测 PA 的无毒且低成本传感器至关重要。本研究通过硅胶柱层析直接从食用酱油中分离出碳点（CDs），设计了一种用于 PA 检测的环保型荧光探针。该 CD 的制备既不需要有机试剂也不需要加热过程。所得的 CDs 具有明亮的蓝色荧光、良好的水溶性和光稳定性。荧光探针的开发依据是 CDs 与 PA 之间的内滤效应会显著猝灭 CD 的荧光。该方法的线性范围为 0.2-24 μM，检出限为 70 nM。该方法成功用于实际水样中 PA 的检测，回收率在 98.0-104.0%之间。此外，由于 CDs 的低毒性和良好的生物相容性，其适用于 HeLa 细胞的荧光成像。
