Mendoza Carmen, Ruiz-Requena Estrella, Ortega Esperanza, Cremades Nieves, Martinez Francisco, Bernabeu Rafael, Greco Ermanno, Tesarik Jan
Department of Biochemistry and Molecular Biology, University of Granada, Granada, Spain.
Hum Reprod. 2002 Apr;17(4):1017-22. doi: 10.1093/humrep/17.4.1017.
Concentrations of certain substances in follicular fluid (FF) are related to fertilization outcome and early post-fertilization development. The study aim was to identify FF markers with which to predict embryo implantation potential.
Concentrations of selected hormones, cytokines and growth factors in individual FF samples obtained during assisted reproduction treatment were related with treatment outcomes.
Mean concentrations of LH, growth hormone (GH), prolactin (PRL), 17beta-estradiol (E2) and insulin-like growth factor (IGF)-I were higher, and that of interleukin-1 (IL-1) was lower, in treatment attempts leading to a clinical pregnancy as compared with those in which no pregnancy was established. Concentrations of FSH, progesterone, tumour necrosis factor-alpha and IGF-II were similar in successful and unsuccessful attempts. In successful attempts, LH and GH levels were higher in those follicles from which oocytes giving rise to transferred embryos (i.e. embryos with best morphology and fastest cleavage rate) originated, as compared with other follicles from which a mature oocyte was recovered but was cryopreserved for later use.
FF levels of LH, GH, PRL, E2, IGF-I and IL-1 may serve to analyse cases of repeated assisted reproduction failures and to assess effects of modifications of the ovarian stimulation protocol.
卵泡液（FF）中某些物质的浓度与受精结果及受精后早期发育相关。本研究旨在确定可用于预测胚胎着床潜能的卵泡液标志物。
将辅助生殖治疗期间获得的单个卵泡液样本中选定激素、细胞因子和生长因子的浓度与治疗结果相关联。
与未成功妊娠的治疗尝试相比，导致临床妊娠的治疗尝试中，促黄体生成素（LH）、生长激素（GH）、催乳素（PRL）、17β-雌二醇（E2）和胰岛素样生长因子（IGF）-I的平均浓度较高，而白细胞介素-1（IL-1）的浓度较低。成功与未成功尝试中，促卵泡生成素（FSH）、孕酮、肿瘤坏死因子-α和IGF-II的浓度相似。在成功的尝试中，与回收成熟卵母细胞但冷冻保存以供后期使用的其他卵泡相比，产生移植胚胎（即形态最佳、分裂速度最快的胚胎）的卵母细胞所来自的卵泡中，LH和GH水平更高。
LH、GH、PRL、E2、IGF-I和IL-1的卵泡液水平可用于分析反复辅助生殖失败的病例，并评估卵巢刺激方案调整的效果。
