Innanen V T, Nicholls D M
Chem Biol Interact. 1975 Nov;11(5):431-9. doi: 10.1016/0009-2797(75)90010-1.
Treatment of rats with the aminonucleoside of puromycin, which increases the incorporation of labelled phenylalanyl-tRNA into polypeptide chains in liver ribosome preparations studied in vitro, did not change the factor-dependent binding of fMet-tRNA f Met to ribosomes nor the peptidyl transferase function of the ribosomes. Peptidyl transferase function, as measured by fMet-tRNA f Met-puromycin formation, was comparable in the free and bound ribosome preparations. Similarly, the factor-dependent binding of fMet-tRNA f Met to ribosomes was the same in free ribosome preparations obtained from rat liver as it was in bound ribosome preparations that had been freed of membranes by puromycin incubation and high salt wash.
用嘌呤霉素氨基核苷处理大鼠，在体外研究的肝核糖体制剂中，嘌呤霉素氨基核苷可增加标记的苯丙氨酰 - tRNA掺入多肽链的量，但这并未改变fMet - tRNAfMet与核糖体的因子依赖性结合，也未改变核糖体的肽基转移酶功能。通过fMet - tRNAfMet - 嘌呤霉素形成来测量的肽基转移酶功能，在游离核糖体制剂和结合核糖体制剂中相当。同样，fMet - tRNAfMet与核糖体的因子依赖性结合，在从大鼠肝脏获得的游离核糖体制剂中，与通过嘌呤霉素孵育和高盐洗涤去除膜的结合核糖体制剂中相同。
