McEwen Nicholls D, Carey J, Sendecki W
Biochem J. 1977 Sep 15;166(3):463-71. doi: 10.1042/bj1660463.
Homogenates of rat liver obtained 3 or 14 days after partial hepatectomy were used to prepare the postmicrosomal pH5-supernatant fraction and to prepare salt-wash fractions of the 40S ribosomal subunits and the 80S ribosomes. The factor-dependent binding of methionyl-tRNAfMet to ribosomes and the elongation-factor-1-dependent binding of phenylalanyl-tRNA to ribosomes were both increased after 3 days of growth, but not after 14 days of growth. An activity inhibitory to phenylalanyl-tRNA binding that was located in ribosomal wash fractions was decreased after 14 days of growth. Since the decreased inhibitory activity was obtained from the ribosomes and was tested against ribosomes and excess of pH5-supernatant fraction from control rat liver, its action was separate from the phenylalanyl-tRNA binding activities of the pH5-supernatant fractions from sham-operated and regenerating liver.
在部分肝切除术后3天或14天获得的大鼠肝脏匀浆,用于制备微粒体后pH5上清液组分,以及制备40S核糖体亚基和80S核糖体的盐洗组分。生长3天后,甲硫氨酰 - tRNAfMet与核糖体的因子依赖性结合以及苯丙氨酰 - tRNA与核糖体的延伸因子 - 1依赖性结合均增加,但生长14天后未增加。位于核糖体洗提组分中的对苯丙氨酰 - tRNA结合有抑制作用的活性在生长14天后降低。由于降低的抑制活性是从核糖体获得的,并且是针对来自对照大鼠肝脏的核糖体和过量的pH5上清液组分进行测试的,其作用与假手术和再生肝脏的pH5上清液组分的苯丙氨酰 - tRNA结合活性是分开的。