Gong Jian-Ping, Liu Chong-An, Wu Chuan-Xin, Li Sheng-Wei, Shi Yu-Jun, Li Xu-Hong
Department of General Surgery, The Second College of Clinical Medicine & the Second Affiliated Hospital of Chongqing University of Medical Science, 74 Linjiang Road, Central District, Chongqing 400010, China.
World J Gastroenterol. 2002 Apr;8(2):346-9. doi: 10.3748/wjg.v8.i2.346.
To determine the NF-kB activity in peripheral blood mononuclear cells (PBMC) in patients with acute cholangitis of severe type (ACST) and correlate the degree of NF-kB activation with severity of biliary tract infection and clinical outcome.
Twenty patients with ACST were divided into survivor group (13 cases) and nonsurvivor group (7 cases). Other ten patients undergoing elective gastrectomy or inguinal hernia repair were selected as control group. Peripheral blood samples were taken 24 hours postoperatively. PBMC were separated by density gradient centrifugation, then nuclear proteins were isolated from PBMC, and Electrophoretic Mobility Shift Assay (EMSA) used determined. The results were quantified by scanning densitometer of a Bio-Image Analysis System and expressed as relative optical density (ROD). The levels of TNF-alpha, IL-6, and IL-10 in the plasma of patients with ACST and healthy control subjects were determined by using an enzyme-linked immunoassay (ELISA).
The NF-kB activity was 5.02 +/- 1.03 in nonsurvivor group, 2.98 +/- 0.51 in survivor group and 1.06 +/- 0.34 in control group. There were statistical differences in three groups (P<0.05). The levels of TNF-alpha and IL-6 in plasma were (498 +/- 53)ng.L(-1)and (587 +/- 64)ng.L(-1)in nonsurvivor group, (284 +/- 32)ng.L(-1) and (318 +/- 49)ng.L(-1)in survivor group and (89 +/- 11)ng.L(-1) and (102 +/-13)ng.L(-1)in control group. All patients with ACST had increased levels of TNF-alpha and IL-6, which were many-fold greater than those of control group, and there was an evidence of significantly higher levels in those of nonsurvivor group than that in survivor group (P<0.05). The levels of IL-10 in plasma were (378+/-32)ng.L(-1), (384+/-37)ng.L(-1) and (68+/-11)ng.L(-1) in three groups, respectively. All patients had also increased levels of IL-10 when compared with control group (P<0.05), but the IL-10 levels were not significantly higher in nonsurvivors than in survivors (P>0.05).
NF-kB activity in PBMC in patients with ACST increases markedly and the degree of NF-kB activation is correlated with severity of biliary tract infection and clinical outcome.
测定重型急性胆管炎（ACST）患者外周血单个核细胞（PBMC）中核因子-κB（NF-κB）的活性，并将NF-κB激活程度与胆道感染严重程度及临床结局相关联。
20例ACST患者分为存活组（13例）和非存活组（7例）。另选10例接受择期胃切除术或腹股沟疝修补术的患者作为对照组。术后24小时采集外周血样本。通过密度梯度离心分离PBMC，然后从PBMC中分离核蛋白，采用电泳迁移率变动分析（EMSA）进行测定。结果通过生物图像分析系统的扫描密度计进行定量，以相对光密度（ROD）表示。采用酶联免疫吸附测定（ELISA）法测定ACST患者和健康对照者血浆中肿瘤坏死因子-α（TNF-α）、白细胞介素-6（IL-6）和白细胞介素-10（IL-10）的水平。
非存活组NF-κB活性为5.02±1.03，存活组为2.98±0.51，对照组为1.06±0.34。三组间差异有统计学意义（P<0.05）。非存活组血浆中TNF-α和IL-6水平分别为（498±53）ng·L⁻¹和（587±64）ng·L⁻¹，存活组为（284±32）ng·L⁻¹和（318±49）ng·L⁻¹，对照组为（89±11）ng·L⁻¹和（102±13）ng·L⁻¹。所有ACST患者TNF-α和IL-6水平均升高，比对照组高许多倍，且非存活组明显高于存活组（P<0.05）。三组血浆中IL-10水平分别为（378±32）ng·L⁻¹、（384±37）ng·L⁻¹和（68±11）ng·L⁻¹。与对照组相比，所有患者IL-10水平也升高（P<0.05），但非存活者的IL-10水平与存活者相比无显著升高（P>0.05）。
ACST患者PBMC中NF-κB活性明显增加，NF-κB激活程度与胆道感染严重程度及临床结局相关。
