Wang Y, Rotem E, Andriani F, Garlick J A
Department of Oral Biology and Pathology, School of Dental Medicine, SUNY at Stony Brook, NY 11794-8702, USA.
J Dent Res. 2001 Sep;80(9):1862-6. doi: 10.1177/00220345010800091801.
Smokeless tobacco is associated with pathologic alterations of the oral mucosa, yet its direct effects on human keratinocytes and fibroblasts in stratified squamous epithelium are not well-understood. We hypothesized that smokeless tobacco could modulate the growth of keratinocytes and fibroblasts in an in vivo-like, organotypic tissue model. To test this, we exposed organotypic cultures for 3 days to smokeless tobacco aqueous extracts and determined the changes in morphology and proliferation of human keratinocytes and fibroblasts. All smokeless tobaccos stimulated keratinocyte proliferation at low doses (0.25% w/v) and suppressed growth at higher doses (> 0.5% w/v). In contrast, smokeless tobacco extracts promoted fibroblast growth at all concentrations without inducing fibroblast turnover. Fibroblasts and keratinocytes, therefore, were differentially affected by smokeless tobacco extracts in an organotypic tissue model, suggesting incipient changes that may occur in vivo.
无烟烟草与口腔黏膜的病理改变有关，但其对复层鳞状上皮中人类角质形成细胞和成纤维细胞的直接影响尚不清楚。我们推测，在类体内的器官型组织模型中，无烟烟草可调节角质形成细胞和成纤维细胞的生长。为验证这一点，我们将器官型培养物暴露于无烟烟草水提取物中3天，并测定人类角质形成细胞和成纤维细胞的形态和增殖变化。所有无烟烟草在低剂量（0.25% w/v）时刺激角质形成细胞增殖，而在高剂量（> 0.5% w/v）时抑制生长。相比之下，无烟烟草提取物在所有浓度下均促进成纤维细胞生长，且不诱导成纤维细胞更新。因此，在器官型组织模型中，成纤维细胞和角质形成细胞受无烟烟草提取物的影响不同，这表明体内可能会出现早期变化。
