Merne Marina, Heikinheimo Kristiina, Saloniemi Irma, Syrjänen Stina
Department of Oral Pathology and Radiology, Institute of Dentistry, University of Turku, Lemminkäisenkatu 2, FIN-20520 Turku, Finland.
Oral Oncol. 2004 Jan;40(1):6-12. doi: 10.1016/s1368-8375(03)00109-x.
Snuff is a locally irritative agent causing hyperkeratinization and hyperplasia of the oral epithelium. The aim of the study was to investigate the effects of snuff on epithelial cell growth and differentiation in vitro. Three-dimensional HaCaT cell cultures were grown for 6, 12, 14, and 18 days in the presence of 1% snuff extract. Ki-67, p53 and cytokeratins (Cks) 5, 13, 10, 19, 18, involucrin and filaggrin were studied by means of immunohistochemistry. Ki-67 indices were assessed, and the results analyzed statistically. Marked morphologic changes were seen with advanced culture time in the snuff group, probably as a result of increased toxic effects. Snuff exposure decreased the percentage of Ki-67 positive cells on days 6, 12, and 14, suggesting that snuff does not stimulate proliferation activity in this in vitro model. Cornification-related Ck 10 decreased after snuff exposure, indicating disturbances in the epithelial differentiation process.
鼻烟是一种局部刺激性物质，可导致口腔上皮的角化过度和增生。本研究的目的是调查鼻烟对体外上皮细胞生长和分化的影响。在含有1%鼻烟提取物的条件下，将三维HaCaT细胞培养6天、12天、14天和18天。通过免疫组织化学研究Ki-67、p53和细胞角蛋白（Cks）5、13、10、19、18、内披蛋白和聚丝蛋白。评估Ki-67指数，并对结果进行统计学分析。在鼻烟组中，随着培养时间的延长，可见明显的形态学变化，这可能是毒性作用增加的结果。在第6天、12天和14天，接触鼻烟降低了Ki-67阳性细胞的百分比，表明在该体外模型中鼻烟不会刺激增殖活性。接触鼻烟后，与角化相关的Ck 10减少，表明上皮分化过程受到干扰。
