Bergersen Linda, Rafiki Amina, Ottersen Ole Petter
Department of Anatomy, Institute of Basic Medical Sciences, University of Oslo, Norway.
Neurochem Res. 2002 Feb;27(1-2):89-96. doi: 10.1023/a:1014806723147.
An efficient exchange of lactate between different cell types (such as astrocytes and neurones) would require that lactate transporters are expressed in contiguous parts of the respective plasma membranes. To settle this issue we explored the subcellular expression pattern of monocarboxylate transporters (MCTs) by use of selective antibodies and high resolution immunogold cytochemistry. We investigated whether the membrane domains containing MCT1, MCT2 and MCT4 are spatially related to each other and to other membrane domains, i.e. those containing glutamate receptors. We used retina and cerebellum as a model for our investigations. We found that MCT1 was localized in the apical membrane of pigment epithelial cells and in the photoreceptor inner segment membrane in the retina. In the brain MCT1 was present in endothelial cells. MCT2 was localized in the postsynaptic membrane of parallel fiber-Purkinje cell synapses and MCT4 was situated in the membrane of glial cells in the cerebellum.
不同细胞类型（如星形胶质细胞和神经元）之间高效的乳酸交换需要乳酸转运体在各自质膜的相邻部分表达。为了解决这个问题，我们通过使用选择性抗体和高分辨率免疫金细胞化学方法，探索了单羧酸转运体（MCTs）的亚细胞表达模式。我们研究了含有MCT1、MCT2和MCT4的膜结构域彼此之间以及与其他膜结构域（即含有谷氨酸受体的膜结构域）在空间上是否相关。我们以视网膜和小脑作为研究模型。我们发现，MCT1定位于视网膜色素上皮细胞的顶端膜以及光感受器内节膜。在大脑中，MCT1存在于内皮细胞中。MCT2定位于平行纤维 - 浦肯野细胞突触的突触后膜，而MCT4位于小脑中神经胶质细胞的膜上。
