Aspenberg Per, Astrand Jörgen
Department of Orthopedics, Linköping University Hospital, Sweden.
Acta Orthop Scand. 2002 Jan;73(1):20-3. doi: 10.1080/000164702317281350.
Bisphosphonates bind to bone surfaces and inactivate osteoclasts when they start to resorb the bone. Therefore, immersion of a bone graft in a bisphosphonate solution before implantation may protect it from resorption. We implanted frozen cancellous bone allografts into bilateral bone chambers for 6 weeks in 10 rats. One graft in each pair had been immersed in an alendronate solution (1 mg/mL) for 10 minutes, and then rinsed in saline. Controls underwent the same treatment with saline only. Results were evaluated with histomorphometry. Control grafts were almost entirely resorbed, but alendronate-treated grafts seemed intact. In the treated specimens, two thirds of the space behind the bone ingrowth frontier consisted of graft or host bone, but in the controls, only one fifth. Local graft treatment with a bisphosphonate before insertion seems to be risk-free, and may prevent mechanical graft failure due to resorption in patients.
双膦酸盐与骨表面结合，并在破骨细胞开始吸收骨时使其失活。因此，在植入前将骨移植体浸泡在双膦酸盐溶液中可能会保护其不被吸收。我们将冷冻的同种异体松质骨移植体植入10只大鼠的双侧骨腔中6周。每对移植体中的一个在阿仑膦酸盐溶液（1mg/mL）中浸泡10分钟，然后用盐水冲洗。对照组仅用盐水进行相同处理。结果用组织形态计量学进行评估。对照移植体几乎完全被吸收，但经阿仑膦酸盐处理的移植体看起来完好无损。在处理过的标本中，骨长入前沿后方三分之二的空间由移植体或宿主骨组成，但在对照组中，仅为五分之一。在植入前用双膦酸盐对移植体进行局部处理似乎没有风险，并且可能防止患者因吸收导致的机械性移植失败。
