Seghatchian J, Krailadsiri P
National Blood Service, London and South East, Colindale, UK.
Transfus Apher Sci. 2002 Feb;26(1):37-41. doi: 10.1016/s1473-0502(01)00143-4.
182 full double donations were collected, using 7-9 cycles with an in-house guideline based on donor HCT, and platelet count. 95.6% of donations were within specifications for platelet yield and 100% were leucodepleted. To overcome the potential for red cell spill-over when the platelet peak is too small, automated continuous monitoring of machine performance has been introduced reducing the potential for spill-over. To understand the impact of the continuous exposure of platelets to the filter, the quality of the first and second part of the collection was monitored using a new set of markers for the platelet storage lesion (activation, microvesiculation, cellular injury, complement and response to ADP). The results were equivalent and compared well with routine practice. Our new protocol allows a wide range of donors to be selected and the highest percentage of products to meet the UK specification with consistency. It is recommended that it can be used for double dose platelets with routine SPM.
共收集了182次全量双份捐献，依据供体造血干细胞移植（HCT）和血小板计数，按照内部指南进行了7 - 9个周期的采集。95.6%的捐献血小板产量符合规格，100%进行了白细胞去除。为克服血小板峰值过小时红细胞溢出的可能性，引入了机器性能的自动连续监测，降低了溢出的可能性。为了解血小板持续接触过滤器的影响，使用一组新的血小板储存损伤标志物（激活、微囊泡形成、细胞损伤、补体和对二磷酸腺苷的反应）监测采集第一部分和第二部分的质量。结果相当，与常规做法相比效果良好。我们的新方案允许选择广泛的供体，并且最高比例的产品能够一致地符合英国规格。建议可将其用于常规单采血小板的双倍剂量血小板采集。
