采用白膜层法在圆柱形袋中制备血小板浓缩物，无需过滤即可实现低白细胞污染。

Low leukocyte contamination without filtration by preparation of platelet concentrates in cylindrical bags with the buffy-coat method.

作者信息

Kostelijk E H, Loos J A, Veldman H A, de Korte D

机构信息

Department of Blood Transfusion Technology, Division CLB of Sanquin Blood Supply Foundation, Amsterdam, The Netherlands.

出版信息

Vox Sang. 2000;79(2):87-93. doi: 10.1159/000031218.

DOI:10.1159/000031218

PMID:11054046

Abstract

BACKGROUND AND OBJECTIVES

The buffy-coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration.

MATERIALS AND METHODS

BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined.

RESULTS

With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 +/- 0.19 versus 3.0 +/- 1.71 x 10(6) leukocytes per unit; 85-ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 +/- 16.4 versus 61 +/- 15 x 10(9) platelets per PC). Furthermore, when PCs were prepared from 100-ml BCs in cylindrical bags, a leukocyte contamination of 0.2 +/- 0.11 x 10(6) and a platelet content of 61 +/- 13.5 x 10(9) per PC were obtained.

CONCLUSION

The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko-depletion without affecting platelet recovery.

摘要

背景与目的

对用于制备血小板浓缩物（PC）的白膜层（BC）法进行了改良，以便从单个白膜层中获得白细胞去除的血小板浓缩物而无需过滤。

材料与方法

将白膜层在圆柱形白膜层袋中进行离心，并确定最佳离心条件和最佳血细胞比容。

结果

在最佳条件下，与传统形状的宽白膜层袋相比，白细胞污染降低了10倍（每单位85毫升白膜层中白细胞数为0.3±0.19对3.0±1.71×10⁶；）。圆柱形袋获得的血小板产量与传统袋获得的产量无显著差异（每个血小板浓缩物中血小板数为56±16.4对61±15×10⁹）。此外，当从圆柱形袋中的100毫升白膜层制备血小板浓缩物时，每个血小板浓缩物的白细胞污染为0.2±0.11×10⁶，血小板含量为61±13.5×10⁹。