Kostelijk E H, Loos J A, Veldman H A, de Korte D
Department of Blood Transfusion Technology, Division CLB of Sanquin Blood Supply Foundation, Amsterdam, The Netherlands.
Vox Sang. 2000;79(2):87-93. doi: 10.1159/000031218.
The buffy-coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration.
BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined.
With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 +/- 0.19 versus 3.0 +/- 1.71 x 10(6) leukocytes per unit; 85-ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 +/- 16.4 versus 61 +/- 15 x 10(9) platelets per PC). Furthermore, when PCs were prepared from 100-ml BCs in cylindrical bags, a leukocyte contamination of 0.2 +/- 0.11 x 10(6) and a platelet content of 61 +/- 13.5 x 10(9) per PC were obtained.
The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko-depletion without affecting platelet recovery.
对用于制备血小板浓缩物（PC）的白膜层（BC）法进行了改良，以便从单个白膜层中获得白细胞去除的血小板浓缩物而无需过滤。
将白膜层在圆柱形白膜层袋中进行离心，并确定最佳离心条件和最佳血细胞比容。
在最佳条件下，与传统形状的宽白膜层袋相比，白细胞污染降低了10倍（每单位85毫升白膜层中白细胞数为0.3±0.19对3.0±1.71×10⁶；）。圆柱形袋获得的血小板产量与传统袋获得的产量无显著差异（每个血小板浓缩物中血小板数为56±16.4对61±15×10⁹）。此外，当从圆柱形袋中的100毫升白膜层制备血小板浓缩物时，每个血小板浓缩物的白细胞污染为0.2±0.11×10⁶，血小板含量为61±13.5×10⁹。
使用圆柱形白膜层袋可将血小板浓缩物中的白细胞污染降低至白细胞去除所需水平，而不影响血小板回收率。
