Reddy Micaela B, Stinchcomb Audra L, Guy Richard H, Bunge Annette L
Chemical Engineering and Petroleum Refining Department, Colorado School of Mines, Golden 80401, USA.
Pharm Res. 2002 Mar;19(3):292-8. doi: 10.1023/a:1014443001802.
Tape stripping the outermost skin layer, the stratum corneum (sc), is a popular method for assessing the rate and extent of dermal absorption in vivo. Results from tape strip (TS) experiments can be affected significantly by chemical diffusion into the sc during the time required to apply and remove all of the TSs, tTS. Here, we examine the effects of this problem on the interpretation of TS experimental results.
Dermal absorption of 4-cyanophenol (4CP) in humans was studied using TS experiments to assess conditions in which diffusion alters TS results. Mathematical models were developed to assess the effects of diffusion on parameter estimation.
For an experiment with tTS > tlag (i.e., the lag time for a chemical to cross the sc), the permeability coefficient for 4CP, P(sc,v), calculated including tTS, was consistent with values from the literature (i.e., 0.0019 cm/h). When diffusion during stripping was not included in the model, P(sc,v) was 70% smaller.
Calculations show that chemical concentrations in TSs can be affected by diffusion during tape stripping, but if tTS < 0.2 tlag and the exposure time is > 0.3 tlag, TS concentrations are not significantly affected by tTS.
胶带剥离最外层皮肤层即角质层（sc），是一种评估体内皮肤吸收速率和程度的常用方法。在粘贴和移除所有胶带条所需的时间tTS内，化学物质向角质层的扩散会显著影响胶带条（TS）实验的结果。在此，我们研究了这个问题对TS实验结果解读的影响。
使用TS实验研究了4-氰基苯酚（4CP）在人体中的皮肤吸收情况，以评估扩散改变TS结果的条件。建立了数学模型来评估扩散对参数估计的影响。
对于一个tTS > tlag（即化学物质穿过角质层的滞后时间）的实验，将tTS考虑在内计算得到的4CP渗透系数P(sc,v)与文献值一致（即0.0019 cm/h）。当模型中不包括剥离过程中的扩散时，P(sc,v)小70%。
计算表明，胶带剥离过程中的扩散会影响胶带条中的化学物质浓度，但如果tTS < 0.2 tlag且暴露时间> 0.3 tlag，则TS浓度不会受到tTS的显著影响。
