Metz Matthew, Fütterer Johannes
Department of Microbiology, University of Washington, Seattle, Washington 98195, USA.
Nature. 2002 Apr 11;416(6881):600-1; discussion 600, 602. doi: 10.1038/nature738. Epub 2002 Apr 4.
Quist and Chapela claim that transgenic DNA constructs have been introgressed into a traditional maize variety in Mexico, and furthermore suggest that these constructs have been reassorted and introduced into different genomic backgrounds. However, we show here that their evidence for such introgression is based on the artefactual results of a flawed assay; in addition, the authors misinterpret a key reference to explain their results, concluding that reassortment of integrated transgenic DNA occurs during transformation or recombination.
奎斯特和查佩拉声称转基因DNA构建体已渗入墨西哥的一个传统玉米品种,并且进一步表明这些构建体已被重新组合并引入不同的基因组背景中。然而,我们在此表明,他们关于这种渗入的证据是基于一种有缺陷检测方法的人为结果;此外,作者错误地解读了一个关键参考文献来解释他们的结果,得出整合的转基因DNA在转化或重组过程中发生重新组合的结论。