Kaplinsky Nick, Braun David, Lisch Damon, Hay Angela, Hake Sarah, Freeling Michael
Department of Plant and Microbial Biology, University of California, Berkeley, California 94720, USA.
Nature. 2002 Apr 11;416(6881):601-2; discussion 600, 602. doi: 10.1038/nature739. Epub 2002 Apr 4.
Quist and Chapela's conclusion that the transgenes they claim to have detected in native maize in Oaxaca, Mexico, are predominantly reassorted and inserted into a "diversity of genomic contexts" seems to be based on an artefact arising from the inverse polymerase chain reaction (i-PCR) they used to amplify sequences flanking 35S transgenes from cauliflower mosaic virus (CaMV).
奎斯特和查佩拉得出结论称，他们声称在墨西哥瓦哈卡州的本地玉米中检测到的转基因主要是重新组合的，并插入到“多种基因组环境”中，这一结论似乎是基于他们用于扩增花椰菜花叶病毒（CaMV）35S转基因侧翼序列的反向聚合酶链反应（i-PCR）所产生的一种假象。
