Mattsson Cecilia, Berggren Diana, Hellström Sten
Department of Internal Medicine, University Hospital of Umeå, Sweden.
Acta Otolaryngol. 2002 Mar;122(2):168-72. doi: 10.1080/00016480252814171.
The aim of this study was to evaluate the development of myringosclerosis in in vitro-cultured tympanic membranes. Sprague-Dawley rats were myringotomized bilaterally and the tympanic membranes were excised after sacrifice. The explants were placed in inserts in wells filled with a nutrient medium. Every second day the tympanic membranes were photodocumented and after 9 days the explants were prepared for histology. On the 4th day the explants had attached to the bottom of the inserts and the specimens had thickened. From the perforation borders and the dissection edges a thin outgrowth was extending across the surface. By Day 9 the perforation had clearly diminished in size when examined in a stereomicroscope. In a light microscope the keratin layer was seen to protrude towards the centre of the perforation and, at the borders, epithelial cells were bridging the gap. Neither the pars tensa nor the pars flaccida showed any sclerotic lesions. The pars flaccida had thickened and the basal cells of the outer keratinized epithelium had invaded the connective tissue. Inflammatory cells were sparse in both the pars tensa and pars flaccida. The in vitro-cultured myringotomized tympanic membrane therefore shows a similar healing pattern to that in vivo. However, inflammatory reactions are sparse and there is no development of myringosclerosis.
本研究的目的是评估体外培养的鼓膜中鼓膜硬化症的发展情况。对Sprague-Dawley大鼠进行双侧鼓膜切开术，处死后切除鼓膜。将外植体置于充满营养培养基的孔板中的小室中。每隔一天对鼓膜进行拍照记录，9天后将外植体准备用于组织学检查。第4天时，外植体已附着于小室底部，标本增厚。从穿孔边缘和解剖边缘有一层薄薄的生长物延伸穿过表面。在体视显微镜下检查时，到第9天时穿孔大小明显减小。在光学显微镜下，可见角质层向穿孔中心突出，在边缘处，上皮细胞桥接了间隙。紧张部和松弛部均未显示任何硬化性病变。松弛部增厚，外层角化上皮的基底细胞侵入结缔组织。紧张部和松弛部的炎性细胞均稀少。因此，体外培养的鼓膜切开术鼓膜显示出与体内相似的愈合模式。然而，炎症反应稀少，且未发生鼓膜硬化症。
