Mattsson C, Stierna P, Hellström S
Department of Otorhinolaryngology, Umeå University Hospital, Sweden.
Am J Otol. 2000 Nov;21(6):804-8.
To attempt to inhibit the development of myringosclerosis by intraperitoneal injection of dexamethasone.
The authors' earlier report showed that the development of myringosclerosis after myringotomy was associated with an inflammatory reaction. The present study was performed to secure evidence for this hypothesis.
Three groups of bilaterally myringotomized rats were treated at 12-hour intervals with intraperitoneal injection of dexamethasone, RU486 (a glucocorticoid receptor antagonist), and saline, respectively. At 6, 12, 24, and 48 hours after the myringotomy, 2 animals were anesthetized on each occasion and examined otomicroscopically. The animals were then killed, and the tympanic membranes were excised and prepared for light microscopic studies.
Dexamethasone treatment retarded and diminished the development of sclerotic lesions markedly. Moreover, no inflammatory signs were seen in the flaccida specimens. When the RU486-treated animals were compared with the animals in the control group, there were no evident differences concerning the development of myringosclerosis or the extent of the inflammatory reaction.
These findings confirm the earlier hypothesis that an inflammatory reaction in collagen tissue is involved in the mechanism that causes the development of myringosclerosis.
通过腹腔注射地塞米松试图抑制鼓膜硬化的发展。
作者早期的报告显示鼓膜切开术后鼓膜硬化的发展与炎症反应有关。本研究旨在为这一假说获取证据。
三组双侧鼓膜切开的大鼠分别每隔12小时接受腹腔注射地塞米松、RU486（一种糖皮质激素受体拮抗剂）和生理盐水治疗。在鼓膜切开术后6、12、24和48小时，每次麻醉2只动物并进行耳显微镜检查。然后处死动物，切除鼓膜并制备用于光镜研究。
地塞米松治疗显著延缓并减轻了硬化病变的发展。此外，在松弛部标本中未观察到炎症迹象。当将接受RU486治疗的动物与对照组动物进行比较时，在鼓膜硬化的发展或炎症反应程度方面没有明显差异。
这些发现证实了早期的假说，即胶原组织中的炎症反应参与了导致鼓膜硬化发展的机制。
