Mattsson C, Johansson C, Hellström S
Department of Otorhinolaryngology, University Hospital of Umeâ, Sweden.
ORL J Otorhinolaryngol Relat Spec. 1999 Jan-Feb;61(1):31-6. doi: 10.1159/000027635.
The aim of the present experimental study was to elucidate the temporal development of myringosclerosis. Twenty-four Sprague-Dawley rats were myringotomized bilaterally. At 3, 6, 9, 12, 18, 24, 30, 36, 48, 60, 72 and 84 h after the myringotomy, 2 animals at each time were examined otomicroscopically and thereafter sacrificed. The pars flaccida and pars tensa were excised and prepared for light- and electron-microscopic studies. Otomicroscopically, myringosclerosis was visible in the pars tensa 24 h after myringotomy, whereas no sclerotic lesions were noted in the pars flaccida. Histologically, sclerotic lesions were present in the pars tensa and pars flaccida 9 and 12 h, respectively, after myringotomy. The pars flaccida responds promptly with an inflammatory reaction characterized by abundant macrophages. Myringosclerosis develops promptly after myringotomy and its establishment is related to an inflammatory reaction.
本实验研究的目的是阐明鼓膜硬化症的时间发展过程。对24只Sprague-Dawley大鼠进行双侧鼓膜切开术。在鼓膜切开术后3、6、9、12、18、24、30、36、48、60、72和84小时，每次取2只动物进行耳显微镜检查，然后处死。切除松弛部和紧张部，准备进行光镜和电镜研究。耳显微镜检查发现，鼓膜切开术后24小时，紧张部可见鼓膜硬化症，而松弛部未发现硬化病变。组织学检查显示，鼓膜切开术后9小时和12小时，紧张部和松弛部分别出现硬化病变。松弛部迅速出现以大量巨噬细胞为特征的炎症反应。鼓膜切开术后鼓膜硬化症迅速发展，其形成与炎症反应有关。
