Chen Luqiu, Koyanagi Madoka, Fukada Kenji, Imanishi Ken'ichi, Yagi Junji, Kato Hidehito, Miyoshi-Akiyama Tohru, Zhang Ruihua, Miwa Keishi, Uchiyama Takehiko
Department of Microbiology and Immunology, School of Medicine, Tokyo Women's Medical University, Ohtsu, Japan.
J Immunol. 2002 Apr 15;168(8):3817-24. doi: 10.4049/jimmunol.168.8.3817.
We analyzed the responses of several T cell fractions reactive with superantigenic toxins (SAGTs), staphylococcal enterotoxin A (SEA), or Yersinia pseudotuberculosis-derived mitogen (YPM) in mice implanted with mini-osmotic pumps filled with SEA or YPM. In mice implanted with the SEA pump, SEA-reactive Vbeta3(+)CD4(+) T cells exhibited a high-level protracted expansion for 30 days, and SEA-reactive Vbeta11(+)CD4(+) T cells exhibited a low-level protracted expansion. SEA-reactive CD8(+) counterparts exhibited only a transient expansion. A similar difference in T cell expansion was also observed in YPM-reactive T cell fractions in mice implanted with the YPM pump. Vbeta3(+)CD4(+) and Vbeta11(+)CD4(+) T cells from mice implanted with the SEA pump exhibited cell divisions upon in vitro restimulation with SEA and expressed surface phenotypes as memory T cells. CD4(+) T cells from mice implanted with the SEA pump exhibited high IL-4 production upon in vitro restimulation with SEA, which was due to the enhanced capacity of the SEA-reactive CD4(+) T cells to produce IL-4. The findings in the present study indicate that, in mice implanted with a specific SAGT, the level of expansion of the SAGT-reactive CD4(+) T cell fractions varies widely depending on the TCR Vbeta elements expressed and that the reactive CD4(+) T cells acquire a capacity to raise a memory response. CD8(+) T cells are low responders to SAGTs.
我们分析了在植入填充有葡萄球菌肠毒素A（SEA）或假结核耶尔森菌衍生丝裂原（YPM）的微型渗透泵的小鼠中，几种与超抗原毒素（SAGTs）、葡萄球菌肠毒素A（SEA）或假结核耶尔森菌衍生丝裂原（YPM）反应的T细胞亚群的反应。在植入SEA泵的小鼠中，SEA反应性Vβ3(+)CD4(+) T细胞持续高水平扩增30天，SEA反应性Vβ11(+)CD4(+) T细胞则持续低水平扩增。SEA反应性CD8(+)对应细胞仅表现出短暂扩增。在植入YPM泵的小鼠的YPM反应性T细胞亚群中也观察到了类似的T细胞扩增差异。植入SEA泵的小鼠的Vβ3(+)CD4(+)和Vβ11(+)CD4(+) T细胞在用SEA进行体外再刺激时表现出细胞分裂，并表达记忆T细胞的表面表型。植入SEA泵的小鼠的CD4(+) T细胞在用SEA进行体外再刺激时表现出高IL-4产生，这是由于SEA反应性CD4(+) T细胞产生IL-4的能力增强。本研究的结果表明，在植入特定SAGT的小鼠中，SAGT反应性CD4(+) T细胞亚群的扩增水平因表达的TCR Vβ元件而异，并且反应性CD4(+) T细胞获得了引发记忆反应的能力。CD8(+) T细胞对SAGTs反应较低。
