[黄芪中关键成分的表征与识别]

[Characterization and recognition key components in Astragalus membranaceus].

作者信息

Zhang Z Z, Liang X M, Zhang Q, Lu P Z

机构信息

Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116011, China.

出版信息

Yao Xue Xue Bao. 2001 Jul;36(7):523-7.

PMID:12585085

Abstract

AIM

To separate, identify and quantify multi-components in complex traditional Chinese medicine (TCM) Astragalus membranaceus by unified analysis for systematic study of its relative contents.

METHODS

The analytical method was reverse-phase HPLC/photodiode array detector. Based on the comparison of UV spectra, software of UV database to assist recognition has been established. Key components have been found and recognized in two reference Astragalus membranaceus and three samples from different sources. With the unified analytical project, relative peak heights of key components were recognized and contrasted.

RESULTS

The water-soluble components can be separated by solvent system A, a gradient program with mobile phase of methanol-water. The liposoluble components can be separated with the solvent system B, a gradient program of mobile phase of 2-propanol-acetonitrile-water. UV detection wavelength was set at 202 nm and the flow rate was 1 mL.min-1 on a Hypersil ODS column. Twenty-nine components in the four Astragalus membranaceus were recognized using characteristic parameters of chromatography and UV spectroscopy. All of the 29 key components in two reference samples and three samples from different sources were shown in their chromatograms to be the same. The concentrations of some the key components in different samples were similar while those of the others were different. The chromatographic peaks of the reference Astragalus membranaceus and two samples were matched with each other. Many different fingerprints were found and differences were shown on their chromatograms. Not only there were differences among three samples from different source, but also there were differences in the two reference samples.

CONCLUSION

To find out the key components and recognize them are the basis of quantitative recognition and are very useful when standards are not available. The method of multi-component quantitative recognition in Astragalus membranaceus was developed. The method is convenient and reproducible. It can be widely used for the quality control of the Astragalus membranaceus.

摘要

目的

通过统一分析对复杂中药黄芪中的多成分进行分离、鉴定和定量，以系统研究其相对含量。

方法

分析方法为反相高效液相色谱/光电二极管阵列检测器。基于紫外光谱比较，建立了辅助识别的紫外数据库软件。在两种对照黄芪和三个不同来源的样品中发现并识别了关键成分。通过统一的分析方案，识别并对比了关键成分的相对峰高。

结果

水溶性成分可用溶剂系统A（甲醇 - 水流动相的梯度程序）分离。脂溶性成分可用溶剂系统B（异丙醇 - 乙腈 - 水流动相的梯度程序）分离。在Hypersil ODS柱上，紫外检测波长设定为202 nm，流速为1 mL·min⁻¹。利用色谱和紫外光谱的特征参数识别了四种黄芪中的29种成分。两个对照样品和三个不同来源样品中的所有29种关键成分在色谱图上显示相同。不同样品中一些关键成分的浓度相似，而其他成分的浓度不同。对照黄芪和两个样品的色谱峰相互匹配。发现了许多不同的指纹图谱，其色谱图上显示出差异。不仅不同来源的三个样品之间存在差异，两个对照样品之间也存在差异。