Boysen Lene, Sørensen Per, Larsen Morten, Serup Jørgen, Kristensen Flemming
Department of Clinical Studies, The Roval Veterinary and Agricultural University, Frederiksberg, Denmark.
Am J Vet Res. 2002 Apr;63(4):565-9. doi: 10.2460/ajvr.2002.63.565.
To investigate whether the degree of erythema during an induced erythematous reaction, the histamine skin test reaction, can be assessed objectively by use of chromametry and image analysis of digital photographs.
9 pet dogs (6 Golden Retrievers and 3 yellow Labrador Retrievers).
Histamine phosphate was injected intradermally, and erythema of the wheal reaction was evaluated during the hour that followed. This was done by use of clinical scores, chromametry, and image analysis of digital photographs. Method reproducibility was tested for visual evaluation of printouts of digital photographs and for image analysis of the same photographs.
The coefficient of variation of the technically derived erythema values was < 10%. The reproducibility of image analysis was high and the range of agreement between observers narrow. Using chromametry, it was not possible to differentiate between various degrees of erythema intensity as visually perceived. In contrast, use of image analysis of digital photographs enabled discrimination of slight erythema from moderate and marked erythema. The dynamics of reaction measured by chromametry followed the clinical observation.
Chromametric values are comparable to those obtained by visual inspection. As the result of standardized conditions, chromametry is preferred over digital photography.
研究在诱导红斑反应（组胺皮肤试验反应）过程中，能否通过色度测定法和数码照片的图像分析客观评估红斑程度。
9只宠物狗（6只金毛寻回犬和3只黄色拉布拉多寻回犬）。
皮内注射磷酸组胺，并在随后的1小时内评估风团反应的红斑情况。通过临床评分、色度测定法和数码照片的图像分析来完成评估。对数码照片打印件的视觉评估和对相同照片的图像分析进行了方法重现性测试。
技术得出的红斑值变异系数<10%。图像分析的重现性高，观察者之间的一致性范围窄。使用色度测定法无法像视觉感知那样区分不同程度的红斑强度。相比之下，使用数码照片的图像分析能够区分轻度红斑与中度和重度红斑。色度测定法测量的反应动态与临床观察结果相符。
色度测定值与目视检查获得的值相当。在标准化条件下，色度测定法优于数码摄影。
