Moody Cary A, Pharr G Todd, Murphey Joey, Hughlett Marian B, Weaver Carroll C, Nelson Phillip D, Coats Karen S
Department of Biological Sciences, Mississippi State University, Mississippi State 39762, USA.
J Vet Diagn Invest. 2002 Mar;14(2):113-9. doi: 10.1177/104063870201400204.
The purpose of this study was to determine whether bovine immunodeficiency virus (BIV) is vertically transmitted in naturally infected dairy cattle. Twenty-two dam/calf pairs from a Mississippi Agriculture and Forestry Experiment Station dairy were the study group. Blood samples were collected following delivery of calves, the peripheral blood leukocytes were purified from these samples, and the leukocyte DNA was used in polymerase chain reactions targeting the pol gene region of the BIV provirus. Southern blotting and hybridization were used to confirm the BIV specificity of the amplified fragments. BIV provirus was detected in 14 of 22 calves (64%), demonstrating vertical transmission. Eight of the calves were disqualified from the final interpretation of transplacental transfer because they may have nursed their mothers prior to blood collection, allowing the possibility of lactogenic transfer of the virus. Transplacental transmission of BIV was identified in 6 of 22 calves (27%).
本研究的目的是确定牛免疫缺陷病毒（BIV）是否在自然感染的奶牛中垂直传播。来自密西西比农业和林业试验站奶牛场的22对母犊对作为研究组。在犊牛出生后采集血样，从这些样本中纯化外周血白细胞，并将白细胞DNA用于针对BIV前病毒pol基因区域的聚合酶链反应。采用Southern印迹和杂交技术确认扩增片段的BIV特异性。在22头犊牛中的14头（64%）检测到BIV前病毒，表明存在垂直传播。其中8头犊牛被排除在经胎盘传播的最终解读之外，因为它们可能在采血前已吃过母乳，存在病毒经乳汁传播的可能性。在22头犊牛中的6头（27%）鉴定出BIV经胎盘传播。
