Gonzalez G C, Johnston J B, Nickel D D, Jacobs R M, Olson M, Power C
Department of Clinical Neurosciences, University of Calgary, Alberta.
Can J Vet Res. 2001 Jan;65(1):73-6.
Bovine immunodeficiency virus (BIV) is a lentivirus that causes disease in cattle. Despite the large cattle industry in western Canada, the presence of BIV has not been examined to date. Genomic DNA, derived from semen and buffy coat samples, was analyzed by nested polymerase chain reaction (PCR) using specific primers for the gag, pol, and env genes of BIV. Despite utilizing a procedure that detected a minimum of 10 proviral copies, BIV sequences were not amplified in any of 317 buffy coat and 50 semen samples that were obtained from an archive that included 27 cattle breeds, collected from different sources in Alberta (1980-1999). In the 367 DNA samples examined, there was no evidence of BIV infection, suggesting that the prevalence of BIV infection was very low.
牛免疫缺陷病毒（BIV）是一种可导致牛发病的慢病毒。尽管加拿大西部有庞大的养牛业，但迄今为止尚未对BIV的存在情况进行过检测。使用针对BIV的gag、pol和env基因的特异性引物，通过巢式聚合酶链反应（PCR）对来自精液和血沉棕黄层样本的基因组DNA进行了分析。尽管采用了一种能够检测至少10个前病毒拷贝的方法，但从一个包含27个牛品种、于1980年至1999年从艾伯塔省不同来源收集的样本库中获取的317份血沉棕黄层样本和50份精液样本中，均未扩增出BIV序列。在所检测的367份DNA样本中，没有BIV感染的证据，这表明BIV感染的流行率非常低。
