Burger R A, Nelson P D, Kelly-Quagliana K, Coats K S
Department of Biological Sciences, College of Arts and Sciences, Mississippi State University, MS 39762, USA.
Am J Vet Res. 2000 Jul;61(7):816-9. doi: 10.2460/ajvr.2000.61.816.
To determine whether bovine immunodeficiency virus (BIV) infection could be detected in spermatozoa, blood leukocytes, or semen leukocytes from stud bulls in artificial insemination centers.
30 bulls at 3 artificial insemination centers.
Polymerase chain reaction testing that used 3 sets of primer pairs targeting pol and env regions of the BIV proviral genome was performed on DNA extracted from semen leukocytes, spermatozoa, and blood leukocytes from each bull. Southern blot analysis was performed to increase sensitivity of detection. Western blot analysis of plasma samples was used to detect antibodies against BIV.
BIV provirus was not detected in DNA samples obtained from semen leukocytes, spermatozoa, or blood leukocytes, and antibodies against BIV were not detected.
Contrary to our report of high point prevalence of BIV contamination of semen from a single artificial insemination center, bulls of the study reported here did not appear to be infected. Maximum risk of BIV infection in similar bulls was estimated at 10% with a confidence level of 95%.
确定在人工授精中心的种公牛的精子、血液白细胞或精液白细胞中是否能检测到牛免疫缺陷病毒（BIV）感染。
3个人工授精中心的30头公牛。
对从每头公牛的精液白细胞、精子和血液白细胞中提取的DNA进行聚合酶链反应检测，该检测使用3组针对BIV前病毒基因组的pol和env区域的引物对。进行Southern印迹分析以提高检测的灵敏度。对血浆样本进行蛋白质印迹分析以检测抗BIV抗体。
在从精液白细胞、精子或血液白细胞获得的DNA样本中未检测到BIV前病毒，也未检测到抗BIV抗体。
与我们关于单个人工授精中心精液BIV污染高流行率的报告相反，此处报告的研究中的公牛似乎未被感染。类似公牛中BIV感染的最大风险估计为10%，置信水平为95%。
