Mühling Jörg, Campos Marie E, Sablotzki Armin, Krüll Matthias, Dehne Marius G, Gonther Jens, Weiss Stefan, Fuchs Markus, Hempelmann Gunter
Department of Anaesthesiology and Intensive Care Medicine, Justus-Liebig-University, University Medical Center, Faculty of Medicine, Giessen, Germany.
Clin Chem Lab Med. 2002 Feb;40(2):111-21. doi: 10.1515/CCLM.2002.020.
We have examined the effects of propofol, taurine, and the combination of propofol and taurine on amino acid profiles and the immune function markers superoxide anion (O2-), hydrogen peroxide (H2O2), and released myeloperoxidase (MPO) activity in neutrophils (PMN). Propofol led to significant changes in the dynamic PMN-free amino acid pool. Exogenous taurine significantly reduced PMN neutral amino acid and alpha-aminobutyrate (alpha-aba) as intracellular taurine increased. Incubation with propofol plus taurine resulted in lower intracellular taurine levels and elevated alpha-aba and neutral amino acid concentrations compared to propofol alone. Concerning PMN immune function markers, propofol significantly decreased O2- and H2O2 formation and released MPO. Taurine led to an increased release of MPO and concomitant significantly reduced O2- and H2O2 levels. When propofol and taurine were applied together they appeared to act additively with regard to superoxide and hydrogen peroxide formation. In the case of MPO, taurine neutralized propofol's effects, supporting the idea that MPO activity may be regulated by taurine. We believe therefore that taurine is important for strengthening PMN host defense capability, although the mechanisms are not yet clear. Moreover, taurine appears to act primarily by altering the PMN osmotic balance, while propofol seems to affect PMN amino acid metabolism and/or uptake and release.
我们研究了丙泊酚、牛磺酸以及丙泊酚与牛磺酸联合使用对氨基酸谱和免疫功能标志物超氧阴离子（O2-）、过氧化氢（H2O2）以及中性粒细胞（PMN）中释放的髓过氧化物酶（MPO）活性的影响。丙泊酚导致了无PMN的氨基酸池动态发生显著变化。外源性牛磺酸随着细胞内牛磺酸增加，显著降低了PMN中性氨基酸和α-氨基丁酸（α-aba）。与单独使用丙泊酚相比，丙泊酚加牛磺酸孵育导致细胞内牛磺酸水平降低，α-aba和中性氨基酸浓度升高。关于PMN免疫功能标志物，丙泊酚显著降低O2-和H2O2的生成以及释放的MPO。牛磺酸导致MPO释放增加，同时显著降低O2-和H2O2水平。当丙泊酚和牛磺酸一起使用时，它们在超氧和过氧化氢生成方面似乎具有相加作用。在MPO方面，牛磺酸中和了丙泊酚的作用，支持MPO活性可能受牛磺酸调节的观点。因此，我们认为牛磺酸对于增强PMN宿主防御能力很重要，尽管其机制尚不清楚。此外，牛磺酸似乎主要通过改变PMN的渗透平衡起作用，而丙泊酚似乎影响PMN氨基酸代谢和/或摄取与释放。
