Mühling J, Engel J, Halabi M, Müller M, Fuchs M, Krüll M, Harbach H, Langefeld T W, Wolff M, Matejec R, Welters I D, Menges T, Hempelmann G
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital Giessen and Marburg, Giessen, Federal Republic of Germany.
Amino Acids. 2006 Jul;31(1):11-26. doi: 10.1007/s00726-006-0273-6. Epub 2006 Mar 20.
We have examined the effects of N(omega)-nitro-L-arginine-methylester-hydrochloride [L-NAME; inhibitor of nitric oxide synthase], S-nitroso-N-acetyl-penicillamine [SNAP; nitric oxide donor], alpha-difluoro-methyl-ornithine [DFMO; inhibitor of ornithine decarboxylase] arginine or ornithine as well as the combination of arginine or ornithine with L-NAME, SNAP or DFMO on intracellular free amino- and alpha-keto acid profiles and the immune function markers superoxide anion and hydrogen peroxide generation as well as released myeloperoxidase activity in neutrophils (PMN). Although the underlying mechanisms still remain unclear, we believe from our results that nitric oxide as well as polyamine-dependent pathways are involved in the signal transmission of free radical molecule, beneficial nutritional therapy or maleficient pharmacological stress-induced alterations in PMN nutrient composition. Relevant changes in intragranulocyte free amino- and alpha-keto acid homeostasis and metabolism, especially, may be one of the determinants in PMN nutrition that positively or negatively influences and modulate neutrophil host defence capability and immunocompetence.
我们研究了盐酸N（ω）-硝基-L-精氨酸甲酯[L-NAME；一氧化氮合酶抑制剂]、S-亚硝基-N-乙酰青霉胺[SNAP；一氧化氮供体]、α-二氟甲基鸟氨酸[DFMO；鸟氨酸脱羧酶抑制剂]、精氨酸或鸟氨酸，以及精氨酸或鸟氨酸与L-NAME、SNAP或DFMO的组合对细胞内游离氨基酸和α-酮酸谱、免疫功能标志物超氧阴离子和过氧化氢生成以及中性粒细胞（PMN）中释放的髓过氧化物酶活性的影响。尽管潜在机制仍不清楚，但从我们的结果来看，我们认为一氧化氮以及多胺依赖性途径参与了自由基分子信号传递、有益营养疗法或有害药物应激诱导的PMN营养成分改变。特别是，颗粒内游离氨基酸和α-酮酸稳态及代谢的相关变化可能是PMN营养的决定因素之一，对中性粒细胞宿主防御能力和免疫能力产生正向或负向影响并进行调节。
