Mühling J, Fuchs M, Campos M, Gonter J, Sablotzki A, Engel J, Welters I D, Wolff M, Matejec R, Dehne M G, Menges T, Krüll M, Hempelmann G
Department of Anaesthesiology, Intensive Care Medicine, Pain Therapy, University Hospital, Justus Liebig University, Giessen, Germany.
Amino Acids. 2004 Dec;27(3-4):313-9. doi: 10.1007/s00726-004-0126-0. Epub 2004 Nov 5.
The objective of this study was to determine the effects of ornithine on polymorphonuclear leucocyte (PMN) free amino- and alpha-keto acid profiles, superoxide anion (O2-) generation, hydrogen peroxide (H2O2) formation and released myeloperoxidase activity (MPO). Exogenous ornithine significantly increased PMN asparagine, glutamine, aspartate, glutamate, arginine, citrulline, alanine, alpha-ketoglutarate and pyruvate as intracellular ornithine increased. Concerning PMN immune function markers ornithine increased H2O2-generation and MPO activity while O2- -formation was decreased. We believe therefore that ornithine is important for affecting PMN "susceptible free amino- and alpha-keto acid pool" although the mechanisms are not yet clear. This may be one of the determinants in PMN nutrition considerably influencing and modulating PMN host defense capability.
本研究的目的是确定鸟氨酸对多形核白细胞（PMN）游离氨基酸和α-酮酸谱、超氧阴离子（O2-）生成、过氧化氢（H2O2）形成以及释放的髓过氧化物酶活性（MPO）的影响。随着细胞内鸟氨酸增加，外源性鸟氨酸显著增加了PMN中的天冬酰胺、谷氨酰胺、天冬氨酸、谷氨酸、精氨酸、瓜氨酸、丙氨酸、α-酮戊二酸和丙酮酸。关于PMN免疫功能标志物，鸟氨酸增加了H2O2生成和MPO活性，而O2-形成减少。因此，我们认为鸟氨酸对于影响PMN“敏感的游离氨基酸和α-酮酸池”很重要，尽管其机制尚不清楚。这可能是PMN营养中的决定因素之一，对PMN宿主防御能力有相当大的影响和调节作用。
