Curtis S K, Cowden R R
Histochemistry. 1975 Nov 21;45(2):115-20. doi: 10.1007/BF00495155.
The carbodiimide-2-hydroxy-3-haphthoic acid hydrazide reaction as developed by Geyer (1964) was used without subsequent diazonium coupling as a fluorescent method for the demonstration of carboxyl groups in both proteins and mucosubstances. The topological distribution of the fluorophore was similar to that reported by Geyer. Quantitative microfluorometric studies on cartilage sections revealed differences in detail between emissions in cartilage matrix mucoprotein as compared to the dense connective tissue associated with the perichondrium which consists principally of protein. It would also appear that the primary fluorescent emission of unstained preparations at 450 mm should be useful in microfluorometric determinations of proteins.
盖耶（1964年）开发的碳二亚胺-2-羟基-3-萘甲酸酰肼反应被用作一种荧光方法，用于证明蛋白质和粘液物质中的羧基，且无需后续重氮偶联。荧光团的拓扑分布与盖耶报道的相似。对软骨切片的定量显微荧光测量研究表明，软骨基质粘蛋白的发射与主要由蛋白质组成的软骨膜相关致密结缔组织的发射在细节上存在差异。此外，未染色制剂在450纳米处的初级荧光发射似乎在蛋白质的显微荧光测量测定中很有用。
