[血清瘦素水平的遗传性：一项双胞胎研究]

[Heritability of serum leptin levels: a twin study].

作者信息

Wu Meihua, He Qingbo, Zhu Dingliang, He Xing, Wang Guliang, Gao Pingjing, Zhou Huaifa, Zhang Weizhong, Zhao Guangsheng

机构信息

Ruijin Hospital/Shanghai Institute of Hypertension, Shanghai Second Medical University, Shanghai, 200025 P. R. China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Apr;19(2):112-4.

PMID:11941584

Abstract

OBJECTIVE

To study the contribution of genetic factors to the variance of serum leptin concentration in healthy, normotensive twins.

METHODS

A total of 57 pairs of twins were investigated: 28 female and 19 male pairs of monozygotic(MZ) twins, and 6 female and 4 male pairs of dizygotic(DZ) twins. The zygosity of twins was determined by comparing the concordance of the genotype of nine fluorescence-labeled microsatellite markers. The genetic analysis was performed using the variance-based method. Serum leptin levels were determined in duplicate by a radioimmunoassay Kit (Linco Research, Inc., St. Charles, Missouri) as previously described.

RESULTS

The test of genetic variance revealed a significantly larger within-pair variance of serum leptin in the DZ twins, in comparison with the MZ twins. The corresponding heritability for serum leptin was 8%. Adjusted for BMI, gender, and uric acid (UA), the heritability for serum leptin was 0.18%. Log leptin correlated significantly with blood pressure (SBp r=0.355 P<0.001; DBp r=0.339 P<0.001). Stepwise multiple linear regression analysis revealed that only BMI, gender and UA were linked independently to serum leptin levels(R(2)=0.788, P<0.001).

CONCLUSION

The above data indicate that environmental factors other than genetic factors are important determinants of leptinemia in normal subjects.

摘要

目的

研究遗传因素对健康、血压正常的双胞胎血清瘦素浓度差异的影响。

方法

共调查了57对双胞胎：28对女性同卵（MZ）双胞胎、19对男性同卵双胞胎、6对女性异卵（DZ）双胞胎和4对男性异卵双胞胎。通过比较9个荧光标记微卫星标记的基因型一致性来确定双胞胎的合子性。采用基于方差的方法进行遗传分析。如前所述，使用放射免疫分析试剂盒（Linco Research公司，密苏里州圣查尔斯）对血清瘦素水平进行双份测定。

结果

遗传方差检验显示，与MZ双胞胎相比，DZ双胞胎血清瘦素的配对内方差显著更大。血清瘦素的相应遗传度为8%。经体重指数（BMI）、性别和尿酸（UA）校正后，血清瘦素的遗传度为0.18%。瘦素对数与血压显著相关（收缩压r = 0.355，P < 0.001；舒张压r = 0.339，P < 0.001）。逐步多元线性回归分析显示，只有BMI、性别和UA与血清瘦素水平独立相关（R² = 0.788，P < 0.001）。