Chen Tian-Jiao, Ji Cheng-Ye, Pang Zeng-Chang, Wang Shao-Jie, Hu Yong-Hua, Qin Ying
Institute of Child and Adolescent Health, Peking University, Beijing 100083, China.
Zhonghua Yu Fang Yi Xue Za Zhi. 2004 Jul;38(4):237-9.
To study the contribution of the genes and environment to variation of serum levels of lipids and lipoprotein.
One hundred and forty-three healthy monozygotic (MZ) twin pairs and 93 dizygotic (DZ) ones aged 5 to 19 [with a mean of (11.2 +/- 3.4) years]. Microsatellite polymorphism (STR) was used to diagnose zygosity of twins, and intraclass correlation coefficient method and Falconer formula were performed to investigate heritability of serum lipids and lipoproteins unadjusted or adjusted for age and sex. Logarithmic transformation was used for data with skewed distribution. Influence of relevant physical and biochemical indicators on serum lipids and other components was analyzed with partial coefficients of correlation adjusted for age and sex.
In the twin samples, difference in serum level of triglycerides (TG) between MZ and DZ was not statistically significant with intraclass variation and intraclass correlation. There was significant difference in serum levels of total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), and lipoprotein [Lp(a)] between MZ and DZ, with their heritability estimates of 0.56, 0.55, 0.49 and 0.58 unadjusted, respectively, and 0.63, 0.63, 0.55 and 0.64 adjusted for age and sex, respectively. Serum levels of TC, HDL-C, LDL-C and Lp(a) correlated reversely with age. Serum levels of TC, HDL-C and LDL-C in girls were slightly higher than those in boys. Most indicators for serum levels of lipids and lipoprotein, except for serum level of Lp(a) correlated with body mass index (BMI), body fat ratio, Pelidisi index, and other indexes such as blood pressure, blood sugar, serum level of calcium, adjusted for age and sex.
Serum levels of TC, HDL-C and Lp(a) were influenced more greatly by genetic factors, and serum level of TG was mainly influenced by environmental ones. Levels of blood lipids in children were influenced by age and sex, and correlated with indicators that reflect their body fat and nutritional status.
研究基因和环境对血脂及脂蛋白血清水平变异的影响。
选取143对5至19岁的健康同卵（MZ）双胞胎和93对异卵（DZ）双胞胎[平均年龄为（11.2±3.4）岁]。采用微卫星多态性（STR）诊断双胞胎的合子性，并运用组内相关系数法和Falconer公式研究血脂和脂蛋白未经年龄和性别调整或经调整后的遗传度。对呈偏态分布的数据进行对数转换。采用经年龄和性别调整的偏相关系数分析相关物理和生化指标对血脂及其他成分的影响。
在双胞胎样本中，MZ和DZ之间甘油三酯（TG）血清水平的差异在组内变异和组内相关性方面无统计学意义。MZ和DZ之间总胆固醇（TC）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）和脂蛋白[Lp(a)]的血清水平存在显著差异，其未经调整的遗传度估计值分别为0.56、0.55、0.49和0.58，经年龄和性别调整后分别为0.63、0.63、0.55和0.64。TC、HDL-C、LDL-C和Lp(a)的血清水平与年龄呈负相关。女孩的TC、HDL-C和LDL-C血清水平略高于男孩。除Lp(a)血清水平外，大多数血脂和脂蛋白血清水平指标与体重指数（BMI）、体脂率、佩利迪西指数以及其他指标（如血压、血糖、血钙水平）经年龄和性别调整后相关。
TC、HDL-C和Lp(a)的血清水平受遗传因素影响较大，TG血清水平主要受环境因素影响。儿童血脂水平受年龄和性别的影响，并与反映其体脂和营养状况的指标相关。
