Tang Luying, Ren Zefang, Zhuang Zhixiong, Liu Xiaohe, Su Zulan, Huang Yu
Research Center of Lung Cancer, Sun Yat-sen University of Medical Sciences, Guangzhou, Guangdong, 510630 P. R. China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Apr;19(2):124-6.
To study the distribution of poly(ADP-ribose) polymerase(PARP) pseudogene polymorphism and the association with susceptibility to lung cancer in Chinese people.
The subjects of this study included 63 patients with lung cancer and 82 healthy controls matched in gender and age. Genome DNA was extracted from white blood cells. Products from PCR with a pair of specific primer were electrophoresized in agarose including EB. Under ultraviolet, observation and imaging were performed.
There was no significant difference in genotype between the cases and controls. The frequencies of B allele in cases and controls were 0.095 and 0.116 respectively. Whether there was B allele or not, smoking was a risk factor of lung cancer (P<0.05). As the genotype was AA and AB or BB, smoking OR was 2.28 and 4.83 respectively. Among non-smokers, the risk at lung cancer did not increase in AB or BB genotypes(P=0.202).
Frequency of B allele is relatively lower in Chinese people than in other races. In smokers, B allele may be a susceptible marker of lung cancer, and there is synergistic function between B allele and smoking.
研究聚（ADP - 核糖）聚合酶（PARP）假基因多态性在中国人群中的分布及其与肺癌易感性的关系。
本研究对象包括63例肺癌患者和82例性别、年龄匹配的健康对照。从白细胞中提取基因组DNA。用一对特异性引物进行PCR扩增产物，在含EB的琼脂糖凝胶中电泳。在紫外线下进行观察和成像。
病例组与对照组基因型无显著差异。病例组和对照组B等位基因频率分别为0.095和0.116。无论有无B等位基因，吸烟都是肺癌的危险因素（P<0.05）。当基因型为AA和AB或BB时，吸烟的比值比分别为2.28和4.83。在非吸烟者中，AB或BB基因型患肺癌的风险未增加（P = 0.202）。
中国人B等位基因频率相对低于其他种族。在吸烟者中，B等位基因可能是肺癌的易感标志物，且B等位基因与吸烟之间存在协同作用。
