Zhang Xue-mei, Miao Xiao-ping, Tan Wen, Qu Shi-ning, Sun Tong, Zhou Yi-feng, Lin Dong-xin
Department of Etiology and Carcinogenesis, Cancer Institute, CAMS and PUMC, Beijing 100021, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2005 Dec;27(6):700-3.
To investigate the association between methylentetrahydrofolate reductase (MTHFR) polymorphisms and risk of lung cancer.
Totally 505 cases with lung cancer and 500 frequency-matched controls were genotyped for the MTHFR C677T and A1298C polymorphisms by polymerase chain reaction-restriction fragment length polymorphism methods. The odds ratios (OR) and 95% confidence intervals (CI) were calculated using unconditional logistic regression model. Haplotype frequency was estimated using EH software.
The frequency of the MTHFR C677T allele in cases was significantly higher than that in controls (53.5% vs 44.9%, P < 0.001). Compared with the 677CC genotype, the 677CT and 677TT genotypes were associated with increased risk of lung cancer, with the OR being 1.43 (95% CI, 1.04-1.95) and 2.40 (95% CI, 1.61-3.59), respectively. In addition, a significant difference in the distribution of haplotype frequencies between cases and controls was observed.
Functional polymorphism in MTHFR is associated with increased risk of lung cancer in Chinese population.
探讨亚甲基四氢叶酸还原酶（MTHFR）基因多态性与肺癌风险之间的关联。
采用聚合酶链反应-限制性片段长度多态性方法，对505例肺癌患者和500例频率匹配的对照进行MTHFR C677T和A1298C基因多态性基因分型。使用非条件逻辑回归模型计算比值比（OR）和95%置信区间（CI）。使用EH软件估计单倍型频率。
病例组中MTHFR C677T等位基因频率显著高于对照组（53.5%对44.9%，P<0.001）。与677CC基因型相比，677CT和677TT基因型与肺癌风险增加相关，OR分别为1.43（95%CI，1.04-1.95）和2.40（95%CI，1.61-3.59）。此外，病例组和对照组之间单倍型频率分布存在显著差异。
MTHFR基因功能多态性与中国人群肺癌风险增加相关。
