[荧光探针双杂交技术及其在医学基因芯片中的应用]
[Fluorescence probe two-hybridization technique and its application in medical genechips].
作者信息
Yang Yuan, Zhang Sizhong, Wang Jun, Yong Gang, Xia Qingjie, Wu Shaoqing, Sun Yan
机构信息
Department of Medical Genetics, West China Hospital, Sichuan University, Chengdu, Sichuan 610041 P. R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2002 Apr;19(2):148-51.
OBJECTIVE
To establish a new nucleic acid hybridization detection technique which may be used in medical genechips.
METHODS
The specific DNA fragment was detected by sequential two hybridization of fluorescence probe with template DNA and fixed DNA probe.
RESULTS
Fluorescence probe two-hybridization (FPTH) was applied to genechips for the detection of sex-transmitted pathogens from culture strains, and the results showed that the values of fluorescence density of the positive groups decreased remarkably when compared with those of the negative group. Both the sensitivity and specificity for detecting clinical samples are higher than 90%. There is no need of any additional reagent in hybridization procedure, and the hybridization detection can be accomplished in 40 minutes.
CONCLUSION
The FPTH technique is rapid, simple and reliable, it can also make the clinical detection process completely automatic and integrative.
目的
建立一种可用于医学基因芯片的新型核酸杂交检测技术。
方法
通过荧光探针与模板DNA及固定化DNA探针的顺序两次杂交来检测特定DNA片段。
结果
荧光探针两次杂交（FPTH）应用于基因芯片检测培养菌株中的性传播病原体，结果显示阳性组的荧光密度值与阴性组相比显著降低。检测临床样本的灵敏度和特异性均高于90%。杂交过程无需任何额外试剂，40分钟即可完成杂交检测。
结论
FPTH技术快速、简便、可靠，还可使临床检测过程完全自动化和一体化。
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