[用于鉴定淋病奈瑟菌的生物素化探针的评估]

[Evaluation of a biotinylated probe for identification of Neisseria gonorrhoeae].

作者信息

Bollet C, Grandguillot M, Bouchemal H, Boutefnouchet N, de Micco P

机构信息

Laboratoire de Microbiologie et d'Hygiène Hospitalière, Hpital Salvator, Sainte-Marguerite, MARSEILLE, France.

出版信息

Pathol Biol (Paris). 1989 Oct;37(8):897-900.

PMID:2515520

Abstract

We evaluated a DNA biotinylated probe for the rapid identification of Neisseria gonorrhoeae in culture (Ortho Diagnostics System, Raritan, NJ). Twenty-one strains of N. gonorrhoeae, including type strain, 57 strains of other Neisseria species, and 104 strains of other genera were studied. The probe was highly sensitive (100%) and specific (96%). All N. gonorrhoeae strains gave strong signals, and only two cross-reaction were observed with N. lactamica, which has a close genetic relationship to N. gonorrhoeae. Our results indicate that specific recombinant DNA probe should offer a reliable and rapid method for routine diagnosis of Neisseria gonorrhoeae.

摘要

我们评估了一种用于在培养物中快速鉴定淋病奈瑟菌的DNA生物素化探针（Ortho诊断系统，拉里坦，新泽西州）。研究了21株淋病奈瑟菌，包括模式菌株、57株其他奈瑟菌属菌株和104株其他属的菌株。该探针具有高度敏感性（100%）和特异性（96%）。所有淋病奈瑟菌菌株均产生强信号，仅观察到两例假阳性反应，均为与淋病奈瑟菌有密切亲缘关系的乳糖奈瑟菌。我们的结果表明，特异性重组DNA探针应为淋病奈瑟菌的常规诊断提供一种可靠且快速的方法。