Goidl J A, Canaani D, Boublik M, Weissbach H, Dickerman H
J Biol Chem. 1975 Dec 10;250(23):9198-205.
Intact detergent-washed HeLa nuclei contain a population of polyribisomes that were released by exposure to polyanions such as RNA or poly(U). The released material appeared by electron microscopic examination to be particles averaging about 200 to 300 angstroms in diameter. Sedimentation velocity analysis of the released particles indicated that the particles had S20,w values of 75 and 110. The particles stimulated amino acid incorporation in an ascites S-30 or S-100 extract at 2.5 mM Mg2+. Studies with a variety of antibiotics indicated that these polyribosomes were capable of elongating but not initiating protein synthesis. Although these polyribosomes may be of cytoplasmic origin, they appear unique in that agents thought to disperse chromatin are required for their release from the nucleus.
完整的经去污剂洗涤的HeLa细胞核含有一群多核糖体，这些多核糖体可通过暴露于多阴离子（如RNA或聚尿苷酸）而释放出来。经电子显微镜检查，释放出的物质似乎是平均直径约为200至300埃的颗粒。对释放出的颗粒进行沉降速度分析表明，这些颗粒的S20,w值分别为75和110。在2.5 mM Mg2+存在的情况下，这些颗粒可刺激腹水S-30或S-100提取物中的氨基酸掺入。对多种抗生素的研究表明，这些多核糖体能够进行蛋白质合成的延伸，但不能起始蛋白质合成。尽管这些多核糖体可能起源于细胞质，但它们的独特之处在于，被认为可分散染色质的试剂是它们从细胞核中释放所必需的。
