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1
Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections.从免疫球蛋白G抗体中快速分离免疫球蛋白M以可靠诊断近期风疹感染。
J Clin Microbiol. 1975 Nov;2(5):377-81. doi: 10.1128/jcm.2.5.377-381.1975.
2
Elution patterns of rubella IgM, IgA, and IgG antibodies from a dextran and an agarose gel.风疹IgM、IgA和IgG抗体从葡聚糖和琼脂糖凝胶上的洗脱模式。
J Clin Pathol. 1975 Aug;28(8):670-3. doi: 10.1136/jcp.28.8.670.
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Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.检测风疹免疫球蛋白M和免疫球蛋白A抗体的快速方法。
J Clin Microbiol. 1975 Feb;1(2):132-5. doi: 10.1128/jcm.1.2.132-135.1975.
4
Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.
Med Microbiol Immunol. 1979 May 15;167(2):77-82. doi: 10.1007/BF02123557.
5
Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.通过琼脂糖凝胶过滤分离风疹IgM、IgA和IgG抗体。
Lancet. 1971 Dec 11;2(7737):1278-80. doi: 10.1016/s0140-6736(71)90600-3.
6
Acute phase serodiagnosis using Quik-Sep ion exchange chromatography.
Am J Clin Pathol. 1985 Apr;83(4):488-91. doi: 10.1093/ajcp/83.4.488.
7
Rapid separation of immunoglobulin M by immunoaffinity chromatography for detection of specific antibodies to rubella and Treponema pallidum.
Eur J Clin Microbiol. 1982 Apr;1(2):118-21. doi: 10.1007/BF02014203.
8
Removal of nonspecific hemagglutination inhibitors, immunoglobulin G, and immunoglobulin A with streptococcal cells and its application to the rubella hemagglutination inhibition test.用链球菌细胞去除非特异性血凝抑制剂、免疫球蛋白G和免疫球蛋白A及其在风疹血凝抑制试验中的应用。
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9
Simple technique for separation of concentrated immunoglobulin M fractions by multi-sample gel chromatography and its application to rubella serodiagnosis.通过多样品凝胶色谱法分离浓缩免疫球蛋白M组分的简单技术及其在风疹血清学诊断中的应用。
J Clin Microbiol. 1981 Feb;13(2):248-51. doi: 10.1128/jcm.13.2.248-251.1981.
10
Antibody responses in patients with rubella infection determined by passive hemagglutination, hemagglutination inhibition, complement fixation, and solid-phase radioimmunoassay tests.通过被动血凝、血凝抑制、补体结合及固相放射免疫测定试验确定的风疹感染患者的抗体反应。
Infect Immun. 1978 Feb;19(2):369-72. doi: 10.1128/iai.19.2.369-372.1978.

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Temporal evolution of human autoantibody response to cytoplasmic rods and rings structure during anti-HCV therapy with ribavirin and interferon-α.在使用利巴韦林和α干扰素进行抗丙型肝炎病毒治疗期间，人类自身抗体对细胞质杆状和环状结构反应的时间演变。
Immunol Res. 2014 Oct;60(1):38-49. doi: 10.1007/s12026-014-8515-2.
2
Protection of mice against tick-borne encephalitis by different classes of immunoglobulins.不同类别的免疫球蛋白对小鼠蜱传脑炎的保护作用。
Infection. 1978;6(4):154-7. doi: 10.1007/BF01641903.
3
The influence of maternal immunoglobulin-G-antibodies on indirect haemagglutination in newborns.母体免疫球蛋白G抗体对新生儿间接血凝反应的影响。
Infection. 1978;6(6):252-6. doi: 10.1007/BF01641981.

本文引用的文献

1
THE TERATOGENICITY OF MATERNAL RUBELLA.孕妇风疹的致畸性。
J Pediatr. 1965 Feb;66:380-91. doi: 10.1016/s0022-3476(65)80196-2.
2
Molecular complexes in the isolation and characterization of plasma lipoproteins.用于分离和鉴定血浆脂蛋白的分子复合物
J Lipid Res. 1961 Apr;2:110-34.
3
Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses.节肢动物传播病毒的血凝及血凝抑制技术。
Am J Trop Med Hyg. 1958 Sep;7(5):561-73. doi: 10.4269/ajtmh.1958.7.561.
4
[The use of pigeon eruthrocytes in the hemagglutination inhibition test for rubella].[鸽红细胞在风疹血凝抑制试验中的应用]
Presse Med (1893). 1967 Oct 21;75(43):2177-8.
5
Rubella-virus hemagglutination-inhibition test.风疹病毒血凝抑制试验
N Engl J Med. 1967 Mar 9;276(10):554-7. doi: 10.1056/NEJM196703092761006.
6
Serological assessment of rubella during pregnancy.孕期风疹的血清学评估。
Br Med J. 1970 Aug 1;3(5717):247-50. doi: 10.1136/bmj.3.5717.247.
7
[Experiences with the rubeola hemagglutination inhibition test].[麻疹血凝抑制试验的经验]
Zentralbl Bakteriol Orig. 1969 Jan;209(2):275-8.
8
Rubella haemagglutination inhibitors: their separation from antibodies.风疹血凝抑制物：它们与抗体的分离
Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):679-85. doi: 10.1111/j.1699-0463.1971.tb00096.x.
9
Single-sample diagnosis of recent rubella by fractionation of antibody on Sephadex G-200 column.通过在葡聚糖凝胶G - 200柱上对抗体进行分级分离对近期风疹进行单样本诊断。
J Clin Pathol. 1971 Sep;24(6):547-50. doi: 10.1136/jcp.24.6.547.
10
Measurement of rubella antibody by hemagglutination inhibition. II. Characteristics of an improved HAI test employing a new method for the removal of non-immunoglobulin HA inhibitors from serum.
J Immunol. 1970 Apr;104(4):826-34.

从免疫球蛋白G抗体中快速分离免疫球蛋白M以可靠诊断近期风疹感染。

Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections.

作者信息

Frisch-Niggemeyer W

出版信息

J Clin Microbiol. 1975 Nov;2(5):377-81. doi: 10.1128/jcm.2.5.377-381.1975.

DOI:10.1128/jcm.2.5.377-381.1975
PMID:1194404
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274194/
Abstract

Chromatography on controlled pore glass was adapted for the separation of immunoglobulin M (IgM) and immunoglobulin G (IgG) rubella antibodies from 0.3-ml samples of human serum. An extremely sharp separation of IgM from IgG antibodies could be obtained within 40 min. Nonspecific inhibitors were removed before chromatography by precipitation with high-molecular-weight dextran sulfate, and the titer of rubella antibodies in the different classes of immunoglobulins were assayed with a modified hemagglutination inhibition technique. The combination of these methods is recommended for routine tests. It permits an accurate diagnosis of recent rubella infection within a few hours.

摘要

采用可控孔径玻璃色谱法从0.3毫升人血清样本中分离免疫球蛋白M（IgM）和免疫球蛋白G（IgG）风疹抗体。在40分钟内可实现IgM与IgG抗体的极清晰分离。在进行色谱分析之前，通过用高分子量硫酸葡聚糖沉淀去除非特异性抑制剂，并采用改良的血凝抑制技术检测不同类免疫球蛋白中风疹抗体的滴度。推荐将这些方法结合用于常规检测。它能在数小时内准确诊断近期风疹感染。