从免疫球蛋白G抗体中快速分离免疫球蛋白M以可靠诊断近期风疹感染。
Rapid separation of immunoglobulin M from immunoglobulin G antibodies for reliable diagnosis of recent rubella infections.
作者信息
Frisch-Niggemeyer W
出版信息
J Clin Microbiol. 1975 Nov;2(5):377-81. doi: 10.1128/jcm.2.5.377-381.1975.
Abstract
Chromatography on controlled pore glass was adapted for the separation of immunoglobulin M (IgM) and immunoglobulin G (IgG) rubella antibodies from 0.3-ml samples of human serum. An extremely sharp separation of IgM from IgG antibodies could be obtained within 40 min. Nonspecific inhibitors were removed before chromatography by precipitation with high-molecular-weight dextran sulfate, and the titer of rubella antibodies in the different classes of immunoglobulins were assayed with a modified hemagglutination inhibition technique. The combination of these methods is recommended for routine tests. It permits an accurate diagnosis of recent rubella infection within a few hours.
摘要
采用可控孔径玻璃色谱法从0.3毫升人血清样本中分离免疫球蛋白M（IgM）和免疫球蛋白G（IgG）风疹抗体。在40分钟内可实现IgM与IgG抗体的极清晰分离。在进行色谱分析之前，通过用高分子量硫酸葡聚糖沉淀去除非特异性抑制剂，并采用改良的血凝抑制技术检测不同类免疫球蛋白中风疹抗体的滴度。推荐将这些方法结合用于常规检测。它能在数小时内准确诊断近期风疹感染。
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