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1
Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.检测风疹免疫球蛋白M和免疫球蛋白A抗体的快速方法。
J Clin Microbiol. 1975 Feb;1(2):132-5. doi: 10.1128/jcm.1.2.132-135.1975.
2
A rountine diagnostic test for IgA and IgM antibodies to rubella virus: absorption of IgG with Staphylococcus aureus.风疹病毒IgA和IgM抗体的常规诊断试验：用金黄色葡萄球菌吸收IgG
J Infect Dis. 1974 Sep;130(3):268-73. doi: 10.1093/infdis/130.3.268.
3
Solid-phase radioimmunoassay of rubella virus immunoglobulin M antibodies: comparison with sucrose density gradient centrifugation test.风疹病毒免疫球蛋白M抗体的固相放射免疫测定：与蔗糖密度梯度离心试验的比较
J Clin Microbiol. 1977 Mar;5(3):257-62. doi: 10.1128/jcm.5.3.257-262.1977.
4
Prolonged rubella IgM antibody response.风疹IgM抗体反应延长。
Med J Aust. 1974 Oct 26;2(17):629-31.
5
Solid-phase radioimmunoassay of rubella virus immunoglobulin G and immunoglobulin M antibodies.风疹病毒免疫球蛋白G和免疫球蛋白M抗体的固相放射免疫测定
J Clin Microbiol. 1976 Aug;4(2):117-23. doi: 10.1128/jcm.4.2.117-123.1976.
6
Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.
Med Microbiol Immunol. 1979 May 15;167(2):77-82. doi: 10.1007/BF02123557.
7
Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.通过琼脂糖凝胶过滤分离风疹IgM、IgA和IgG抗体。
Lancet. 1971 Dec 11;2(7737):1278-80. doi: 10.1016/s0140-6736(71)90600-3.
8
[Studies on specific immunoglobulin responses in serum after spontaneous infection with rubella virus. I. Detection of rubella IgM and IgA hemagglutination inhibition antibodies (author's transl)].
Uirusu. 1978 Dec;28(2):87-94. doi: 10.2222/jsv.28.87.
9
Hemadsorption immunosorbent technique for determination of rubella immunoglobulin M antibody.用于测定风疹免疫球蛋白M抗体的血细胞吸附免疫吸附技术
J Clin Microbiol. 1981 Mar;13(3):410-5. doi: 10.1128/jcm.13.3.410-415.1981.
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Rubellavirus-specific IgM- and IgA-antibodies. The indirect immunofluorescence (IF)-technique applied to sera with reduced IgG-concentration.风疹病毒特异性IgM和IgA抗体。将间接免疫荧光（IF）技术应用于IgG浓度降低的血清。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Oct;82B(5):742-4. doi: 10.1111/j.1699-0463.1974.tb00244.x.

引用本文的文献

1
Hemadsorption immunosorbent technique for determination of rubella immunoglobulin M antibody.用于测定风疹免疫球蛋白M抗体的血细胞吸附免疫吸附技术
J Clin Microbiol. 1981 Mar;13(3):410-5. doi: 10.1128/jcm.13.3.410-415.1981.
2
Rheumatoid factor in syphilis.梅毒中的类风湿因子。
J Clin Microbiol. 1985 Jul;22(1):89-94. doi: 10.1128/jcm.22.1.89-94.1985.
3
Sensitivity and specificity of viral immunoglobulin M determination by indirect enzyme-linked immunosorbent assay.间接酶联免疫吸附测定法检测病毒免疫球蛋白M的敏感性和特异性
J Clin Microbiol. 1988 Feb;26(2):328-32. doi: 10.1128/jcm.26.2.328-332.1988.
4
Predominance of immunoglobulin G sub-class 3 among the complement-fixing antibodies to streptococcal M-associated protein.针对链球菌M相关蛋白的补体结合抗体中免疫球蛋白G 3亚类占优势。
Clin Exp Immunol. 1979 Aug;37(2):247-58.
5
IgA antibody response in acute rubella determined by solid-phase radioimmunoassay.通过固相放射免疫测定法测定急性风疹中的IgA抗体反应。
J Hyg (Lond). 1979 Aug;83(1):69-75. doi: 10.1017/s0022172400025833.
6
Simple method to detect virus-specific IgM antibodies in patients' serum samples after immunosorption of immunoglobulins G and A.
Med Microbiol Immunol. 1979 May 15;167(2):77-82. doi: 10.1007/BF02123557.

本文引用的文献

1
Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
Nature. 1967 Jun 24;214(5095):1302-4. doi: 10.1038/2141302a0.
2
Immunochemical quantitation of antigens by single radial immunodiffusion.通过单向辐射免疫扩散法对抗原进行免疫化学定量。
Immunochemistry. 1965 Sep;2(3):235-54. doi: 10.1016/0019-2791(65)90004-2.
3
IgG, IgA and IgM responses in acute rubella determined by the immunofluorescent technique.用免疫荧光技术测定急性风疹中的IgG、IgA和IgM反应。
J Hyg (Lond). 1972 Sep;70(3):473-85. doi: 10.1017/s0022172400063063.
4
IgM antibodies to Epstein-Barr virus in infectious mononucleosis.传染性单核细胞增多症中针对EB病毒的IgM抗体。
Arch Gesamte Virusforsch. 1972;37(4):332-9. doi: 10.1007/BF01241456.
5
Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence.通过免疫荧光法测定不同的巨细胞病毒免疫球蛋白（IgG、IgA、IgM）
Arch Gesamte Virusforsch. 1972;37(1):131-40. doi: 10.1007/BF01241158.
6
Demonstration of rubella IgM antibody by indirect fluorescent antibody staining, sucrose density gradient centrifugation and mercaptoethanol reduction.通过间接荧光抗体染色、蔗糖密度梯度离心和巯基乙醇还原法检测风疹IgM抗体。
Intervirology. 1973;1(1):48-59. doi: 10.1159/000148832.
7
Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.风疹：一种通过检测IgM抗体来快速诊断近期感染的方法。
Br Med J. 1968 Jan 27;1(5586):221-3. doi: 10.1136/bmj.1.5586.221.
8
Separation of rubella IgM, IgA, and IgG antibodies by gel filtration on agarose.通过琼脂糖凝胶过滤分离风疹IgM、IgA和IgG抗体。
Lancet. 1971 Dec 11;2(7737):1278-80. doi: 10.1016/s0140-6736(71)90600-3.

检测风疹免疫球蛋白M和免疫球蛋白A抗体的快速方法。

Rapid method to detect rubella immunoglobulin M and immunoglobulin A antibodies.

作者信息

Schmitz H, Shimizu H, Kampa D, Doerr H W

出版信息

J Clin Microbiol. 1975 Feb;1(2):132-5. doi: 10.1128/jcm.1.2.132-135.1975.

DOI:10.1128/jcm.1.2.132-135.1975
PMID:1176596
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC274987/
Abstract

Immunoglobulin (Ig) G was removed from serum specimens by precipitation with gamma chain-specific anti-human IgG of rabbit origin. The remaining rubella virus-specific IgM (and IgA) antibodies were then detected by the rubella hemagglutination-inhibition test. This procedure has proven to be as reliable as estimations carried out with IgM fractions separated on a sucrose density gradient.

摘要

通过用兔源γ链特异性抗人IgG沉淀，从血清标本中去除免疫球蛋白（Ig）G。然后通过风疹血凝抑制试验检测剩余的风疹病毒特异性IgM（和IgA）抗体。该方法已被证明与用蔗糖密度梯度分离的IgM组分进行的检测一样可靠。