Romanovskaia V A, Sokolov I G, Malashenko Iu R, Krishtab T P
Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, Kyiv.
Mikrobiol Z. 2002 Jan-Feb;64(1):48-56.
It was established, that EDTA (1.0 mM) and formamide (100 mM) are inhibitors of methanol dehydrogenase in Methylobacter luteus 12b, Methylomonas rubra 15sh and Methylococcus thermophilus 111p. The investigated strains co-metabolised ethane with the use of formate as the co-substrate. The application of formamide (or EDTA) as inhibitors of methanol dehydrogenase prevented from further transformation of ethanol and resulted in accumulation of extracellular ethanol. It was shown, that M. rubra 15sh accumulated extracellular ethanol under cultivation in a chemostate. The carried out researches have shown a regulation path of co-metabolism process of hydrocarbons by methane utilizing bacteria. Using the specific inhibitors of methanol dehydrogenase and a source of reducing agent (energy) for methane monooxygenase with the help of the cells of methane-oxidizing bacteria it is possible to obtain from ethane or other hydrocarbons the products of their monooxygenation--alcohols.
已确定，乙二胺四乙酸（1.0 mM）和甲酰胺（100 mM）是黄色甲基杆菌12b、红色甲基单胞菌15sh和嗜热甲基球菌111p中甲醇脱氢酶的抑制剂。所研究的菌株利用甲酸盐作为共底物与乙烷进行共代谢。应用甲酰胺（或乙二胺四乙酸）作为甲醇脱氢酶的抑制剂可阻止乙醇的进一步转化，并导致细胞外乙醇的积累。结果表明，红色甲基单胞菌15sh在恒化器培养条件下积累细胞外乙醇。所进行的研究揭示了甲烷利用细菌对烃类共代谢过程的调控途径。借助甲烷氧化细菌的细胞，利用甲醇脱氢酶的特异性抑制剂和甲烷单加氧酶的还原剂（能量）来源，有可能从乙烷或其他烃类中获得它们的单加氧产物——醇类。
